中文摘要：

基于传统的实验方法,对胜利油田滩涂区土壤中石油降解菌进行了筛选和鉴定,并利用PCR-DGGE（变性梯度凝胶电泳）技术分析了胜利油田滩涂区的菌群多样性.结果表明：由研究区土壤中筛选出13株石油降解菌,其中,6株石油降解菌的石油降解率〉90%,能降解大部分C12~C26的石油烷烃,系统发育学鉴定为Alcanivorax、Halomonas和Marinobacter,均属于-γproteobacteria分支;胜利油田滩涂区未培养优势菌有Sulfurovum、Gillisia和Arcobacter;该区优势菌群中-γproteobacteria所占比重较大,其次为α-proteobactiria、-εproteobactiria、Acti-nobacteria和Flavobacteria.

英文摘要：

The petroleum-degrading bacteria in Shengli Oil Field wetland soil were isolated and identified by traditional experiment methods, and their diversity was analyzed by PCR-DGGE （denaturing gradient gel electrophoresis）. A total of thirteen petroleum-degrading bacterial strains were isolated, among which, six strains were found to have the ability of degrading the majority of C12-C26 petroleum hydrocarbon, with a degradation rate of 〉 90%. These petroleum degraders were phylogenetiely identified as the members of Halomonas, AIcanivorax, and Marinobacter, which were all belonged to 3,-proteobacteria. The uncultured predominant bacteria in Shengli Oil Field wetland soil were of Sulfurovum, GiUisia and Arcobacter. Among the predominant bacteria, γ-proteobacteria accounted for a larger proportion, followed by ε-proteobactiria＇, e-proteobactiria, Actinobacteria, and Flavobacteria.