中文摘要：

目的观察内毒素诱导的大鼠急性前葡萄膜炎（EIU）虹膜组织内Toll样受体4（TLR4）、髓样分化因子88（MyD88）及核因子-κBp65（NF-κB p65）的表达。方法Wistar大鼠50只，随机分为5组，0、12、24、48、72h，每组10只。0h组为正常对照组，其余4组均足垫部注射霍乱弧菌内毒素脂多糖（LPS）200μg，建立EIU动物模型，每隔2h用裂隙灯观察大鼠眼前节炎症反应。通过铺片免疫组织化学染色，检测虹膜睫状体组织内TLR4、MyD88和NF—κB p65的表达，并对虹膜内TLR4^＋和MyD88^＋及NF—κB p65^＋细胞进行计数。结果注射后24～48h大鼠眼前段的炎症反应达到高峰，72h炎症反应逐渐缓解。组织病理学检查表明，虹膜睫状体组织的炎性细胞浸润在24～48h达到高峰，与临床反应结果相符。TLR4在模型鼠虹膜睫状体炎复合体中表达的免疫组织化学检测结果表明，0h组虹膜铺片内无阳性细胞，12h后可见细胞形态大多为类圆形的阳性细胞，48h达高峰，72h阳性细胞数开始减少，各组阳性细胞数总体差异有统计学意义（F=46．79，P〈0．05）。MyD88和NF—κB p65的表达与TLR4的改变趋势相一致（F=54．37，P〈0．05；F=85．32，P〈0．05）。结论内毒素诱导的EIU虹膜内，TLR4及其下游信号传导分子的表达量发生改变，提示TLR4-MyD88依赖传导途径可能参与了EIU的发病.

英文摘要：

Background Research determined that TLR4 is positively expressed on the macrophages in iris and ciliary in acute endotoxin-induced uveitis（EIU） ,indicating that TLR4 participated in the pathogenesis of the anterior uveitis. Objective The aim of this study is to observe the expressions of toll-like receptor-4 （TLR4）, Myeloid differentiation factor 88 （MyD88）, NF- κB p65 in iris tissue in the eyes with endotoxin-induced acute anterior uveitis. Methods Animal models of acute anterior uveitis were established by a hind footpad injection of 200μg Cholera vibrio LPS in 40 SPF Wistar rats with the age of 6 - 8 weeks. Other 10 age-matched rats were as normal controls. Ocular inflammation was examined under the slit lamp microscope at the 2-hour interval after the injection and intensity of inflammation was scored according to the standard of Lajavardi. Histopathology examination was performed for the evaluation of inflammatory reaction of iris and ciliary tissues by HE staining at 24 hours after LPS injection. Expressions of TLR4, MyD88 and NF-κB p65 in iris and ciliary body tissue were detected through immunohistochemistry. TLR4^＋, MyD88 ^＋ and NF-κB p65 ＋ cells were counted. Results The inflammatory reaction was gradually enhanced after injection of LPS and peaked at 24 hours and allivated 48 hours later. The infiltration of lots of inflammatory cells and fibrinous exudate were exhibited in the anterior chamber, posterior chamber,iris and ciliary tissue under the optical microscope at 24 hours after injection of LPS. No positive expressions of TLR4,MyD88 and NF-κB p65 in iris-ciliary body complex were found in normal control rats. The positive cells for TLR4, MyD88 and NF-κB p65 in iris-ciliary body complex were significantly different among 12 hours group ,48 hours group and 72 hours groups （ F = 46.79, P 〈 0.05 ; F = 54.37, P 〈 0. 05 ; F = 85.32, P 〈 0.05 ） , and the positive cells for TLR4, MyD88 and NF-κB p65 peaked at 24 hours after injection of LPS. Conclusion The expression of