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pre-miR-196a2(rs11614913)、pre-miR-146a(rs2910164)基因多态性与中国皖南地区汉族人群支气管哮喘的相关性
  • ISSN号:1001-7550
  • 期刊名称:牡丹江医学院学报
  • 时间:2015
  • 页码:1-5
  • 分类:R562.25[医药卫生—呼吸系统;医药卫生—临床医学;医药卫生—内科学]
  • 作者机构:[1]皖南医学院,安徽芜湖241002, [2]弋矶山医院,安徽芜湖241002
  • 相关基金:国家自然科学基金资助项目(81270091,30872361,81172790)
  • 相关项目:微小RNA(miRNA)对实验性哮喘CD4+ T细胞增殖和分化的调节机制研究
中文摘要:

目的:探讨pre-miR-196a2(rs11614913)和pre-miR-146a(rs2910164)的基因多态性与中国皖南地区汉族人群支气管哮喘的相关性。方法:选择来自皖南地区的汉族支气管哮喘患者591例及健康对照者621例,采用PCR及飞行时间质谱分型法(MALDI-TOF MS)分析比较两组的基因型、等位基因频率。结果:两组pre-miR-1962(rs11614913)位点等位基因T和C的分布相比,χ2=12.785,p〈0.01,TT、TC、CC基因型分布相比,χ2=13.446,p〈0.01;相对于TT基因型,CC、CT、TC+CC基因型的OR(95%CI)分别为1.647(p〈0.01)、1.500(p〈0.01)和1.542(p〈0.01),表明该位点的多态性可能增加支气管哮喘的危险性。两组pre-miR-146a(rs2910164)位点等位基因G和C的分布相比,χ2=15.766,p〈0.01,CC、CG、GG基因型分布相比,χ2=58.545,p〈0.01;相对于GG基因型,CC、CG、CC+CG基因型的OR(95%CI)分别为2.175(p〈0.01)、3.283(p〈0.01)和2.755(p〈0.01),表明该位点的多态性可能增加支气管哮喘发生的危险性。结论:pre-miR-196a T/C(rs11614913)和pre-miR-146a G/C(rs2910164)位点的多态性可能与中国皖南地区汉族人群支气管哮喘的发生有相关性。

英文摘要:

Objective: To investigate the link between variants in pre- microRNAs and the risk of asthma in a Chinese Han population from Wannan area of Anhui province,including pre- mir- 196a( rs11614913) and pre- mir- 146a( rs2910164). Methods: A case- control study was conductedd to evaluate the effects of pre- mir- 196a( rs11614913) and pre- mir- 146a( rs2910164) on the risk of asthma. A total of 591 cases and 621 controls were recruited for this study. The genotypes were determined using polymerase chain reaction( PCR) and matrix assisted laser desorption / ionization time- of- flight mass spectrometry( MALDI- TOF MS). The frequencies of genotypes and alleles of these SNPs in the cases were also analyzed by logistic repression analysis,compared with those of the controls. Results: For pre- mir- 196 a gene in loci of rs11614913,the alleles frequency T with C of the gene in cases and controls have a significant difference( χ2 = 12. 785,p 〈 0. 01),there was also a significant difference in the distributions of the genotypes( TT,TC,and CC) between asthmatic subjects and normal control( χ2 = 13. 446,p = 0. 01). Logistic regression analysis revealed that the CC,CT,and CT + CC genotypes were significantly increased with the risk of asthma( CC vs. TT,OR( 95% CI) = 1.647( 1. 195- 2. 269),p 〈 0. 01; CT vs. TT,OR( 95% CI) = 1. 500( 1. 165- 1. 931),p 〈 0. 01; CT + CC vs. TT,OR( 95%CI) = 1. 542( 1. 219- 1. 951),p 〈 0. 01),compared with TT genotype. However,integrating data from published literature on an SNP of pre- mir- 196a2( rs11614913) and risk of asthma gives converse results. For pre- mir- 146 a gene in loci of rs2910164 the alleles frequency G with C of the gene in asthmatic and control group have a significant difference( χ2 = 15. 766,p 〈 0. 01),there was also a significant difference in the distributions of the genotypes( CC,CG,and GG) between asthmatic subjects and normal control( χ2 = 58. 545,p 〈 0. 001); Logistic reg

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期刊信息
  • 《牡丹江医学院学报》
  • 主管单位:黑龙江省教育厅
  • 主办单位:牡丹江医学院
  • 主编:朱晓峰
  • 地址:黑龙江省牡丹江市爱民区通乡街3号
  • 邮编:157000
  • 邮箱:mdjyxyxb@126.com
  • 电话:0453-6984206 6984665
  • 国际标准刊号:ISSN:1001-7550
  • 国内统一刊号:ISSN:23-1270/R
  • 邮发代号:14-283
  • 获奖情况:
  • 国内外数据库收录:
  • 被引量:8697