RNA扩增是伴随着基因芯片技术的应用而发展起来的一项技术,但不仅仅应用于芯片杂交。文章对RNA扩增的技术方法进行了综述,重点介绍了mRNA的线性扩增、指数扩增以及两者相结合的扩增方法以及优缺点,对microRNA的扩增方法也作了介绍,并对将来的发展作了展望。
The technology of RNA amplification is developing with the application of gene chip and it is not only adapted for chip hybridization, but also applied in other purposes as well. The methodologies of RNA amplification were reviewed, focused on linear, exponential and linear plus exponential amplifications. The amplification of microRNA was also introduced and a prospect of RNA amplification was made.