中文摘要：

[目的]克隆棉铃虫多核衣壳型多角体病毒（HearMNPV） fp25 K基因,为深入研究杆状病毒的进化机制提供依据。[方法]通过半补齐法建立HearMNPV的质粒基因组文库,对插入片段进行鉴定和序列分析获取HearMNPV fp25 K基因。[结果]HearMNPV fp25 K基因的读码框由588个核苷酸组成,编码195个氨基酸;该基因上游具有晚期调控保守序列ATAAG,是一个晚期表达基因;HearMNPV fp25 K存在5个α-螺旋,8个β-折叠;HearMNPV与蓓带夜蛾核型多角体一致性最高,达97.0%,与舞毒蛾多核衣壳型多角体的FP’同源性最低,仅为19.3%。HearMNPV与GroupⅠNPVs、GroupⅡNPVs及GVs的fp25K同源基因间氨基酸平均相似性分别为55.6%、69.4%和29.3%,推测其与Ⅱ类NPVs的亲缘关系较近。[结论]该研究为杆状病毒分子进化、生物防治奠定了基础。

英文摘要：

[Objective] The aim was to clone the Helicoverpa armigera multiple nucleocapsid nucleopolyhedrovirus（HearMNPV） fp25 K gene in order to provide the basis for studying the evolutionary mechanism of baculovirus deeply.[Method] A plasmid genomic library of HearMNPV was constructed by ＂partial filling-in＂ method,the HearMNPV fp25 K gene was obtained by identifying and analyzing the sequence of the inserted segment.[Result] The reading frames of HearMNPV fp25 K gene was composed of 588 nucleotide acid,encoding 195 amino acids.It had a late gene motif ATAAG and behaved to all baculovirus late genes.HearMNPV fp25 K had 5α-helix and 8 β-sheet.HearMNPV had the highest homology with Mamestra configurata NPV,being 97.0% and had the lowest homology with Lymantria dispar multinucleocapsid NPV,being only 19.3%.The average similarity of amino acid of fp25 K homologous genes between HearMNPV and GroupⅡNPVs,GVs were 55.6%,69.4% and 29.3%,resp.,which could be conferred that the HearMNPV had a closer relationship with the Group II NPVs.[Conclusion] The research laid the foundation for the molecular evolution and the biological control of baculovirus.