通过半补齐法建立棉铃虫多核衣壳型多角体病毒A1 HearMNPV—A1(Helicoverpa armigera multiple nucleocapsid nucleopolyhedrovirus-A1)的质粒基因组文库,拼接了一个24553bp的片断,推测含有26个ORFs,与蓓带夜蛾B多角体病毒MaeoNPV—B(Mamestra configurata Nucleopolyhedrovirus—B)进行基因对等图分析,表明HearMNPV基因片断缺失了一段5466bp的序列,与八字地老虎颗粒体病毒XecnGV(Xestia c—nigrum granulovirus)的orf 60~65基因簇氨基酸序列有高度一致性,基因信息分析表明HearMNPV和XecnGV可发生重组,为杆状病毒在自然界中的基因流向提供了更多的信息.
A genomic library of Helicoverpa armigera multiple nucleocapsid nucleopolyhedrovirus ( HearMNPV) is constructed by "partial 26 putative ORFs. Gene parity filling-in" method, and the 24,553 bp fragment is assembled, which containes comparison of HearMNPV fragment arrangement with that of Mamestra configurata Nucleopolyhedrovirus - B ( MacoNPV - B) indicates that it is a 5 466 bp deletion in HearMNPV, which is high homologous with a cluster ORFs of Xestia c-nigrum granulovirus (XecnGV) from orf60 to orf65. The data suggests that there are recombination between HearMNPV and XecnGV. Also, it provides an insight into the gene flow that may be naturally occurring in field populations of baculoviruses.