中文摘要：

目的观察间歇性低氧及持续性低氧对大鼠血压的影响,探讨银杏叶提取物（GBE）对阻塞性睡眠呼吸暂停综合征（OSAS）并发高血压大鼠血压的作用。方法健康雄性SD大鼠50只,随机分为正常对照组、间歇低氧组、间歇低氧GBE组、持续低氧组和持续低氧GBE组,每组10只。实验前后测量大鼠血压变化;苏木精-伊红（HE）染色观察大鼠心肌、肾、腹主动脉的病理变化;检测大鼠血清中丙二醛（MDA）、4-羟基壬烯酸（4-HNE）、低氧诱导因子1α（HIF-1α）的含量,外周脂肪组织中Toll受体4（TLR4）、JNK蛋白及其mRNA表达水平。结果间歇低氧组大鼠血压明显高于正常对照组、持续低氧组和间歇低氧GBE组（均P〈0.01）;与正常对照组比较,间歇低氧组和持续低氧组心肌细胞、肾小管明显水肿,呈水样变性;与间歇低氧组、持续低氧组比较,间歇低氧GBE组和持续低氧GBE组心肌细胞、肾小管水肿显著降低,腹主动脉均无明显病理改变;与正常对照组比较,间歇低氧组、持续低氧组大鼠的血清中MDA、4-HNE、HIF-1α的含量均显著增高（均P〈0.05）,外周脂肪组织中TLR4、JNK蛋白及其mRNA表达水平均显著增高（均P〈0.01）;与间歇低氧组、持续低氧组比较,间歇低氧GBE组、持续低氧GBE组大鼠血清中MDA、4-HNE、HIF-1α的含量均显著降低（P〈0.05）,外周脂肪组织中TLR4、JNK蛋白及其mRNA表达水平均显著降低（P〈0.01）。结论间歇性低氧可以引起大鼠血压升高,而持续低氧则不能;GBE可以抑制OSAS及持续低氧大鼠脂肪组织TLR4、JNK蛋白及mRNA的表达,并降低血清中MDA、4-HNE、HIF-1α的水平,减轻低氧对组织器官的损害。

英文摘要：

Objective To study the effect of intermittent hypoxia and chronic hypoxia on rat blood pressure,and investigate the impact of gingko biloba extracts（ GBE） on obstructive sleep apnea syndrome（ OSAS） with hypertension in rats.Methods Fifty healthy male Sprague-Dawley（ SD） rats were randomly divided into 5 groups（ n = 10） ： normal control（ NC）,intermittent hypoxia（ IH） model control,intermittent hypoxia with GBE administration,chronic hypoxia,and chronic hypoxia with GBE administration groups.The blood pressure was observed before and after experiment.The pathological changes of myocardia,renal artery and abdominal artery were observed by hematoxylin（ HE） staining.The content of MDA,4-HNE and HIF-1α in serum were determined.The protein and mRNA expression of TLR4 and JNK in rat adipose tissue were detected. Results Blood pressure of IH group was significantly higher than that in NC group and hypoxia group（ bothintermittent and chronic,P 0.01）.Compared with NC group,the myocardial cells swelled significantly and kidney tubules presented hydropic degeneration in both IH group and chronic hypoxia group.In intermittent hypoxia with GBE and chronic hypoxia with GBE groups,the myocardial cell and kidney tubules were without swelling and the abdominal artery was pathologicallyun changed compared with those in IH and chronic hypixia group.Compared with NC group,the level of MDA,4-HNE,HIF-1α in serum were remarkably higher in the IH group and chronic hypoxia group（ P〈0.05）,the level of TLR4,JNK protein and the mRNA expression were obviously increased in adipose tissue of the IH and chronic hypoxia groups（ P 〈0.01）. However,the results of them in intermittent hypoxia with GBE intragastric administration group,chronic hypoxia with GBE intragastric administration group were lower compared with IH and chronic hypoxia group（ P〈0.05）.Compared with NC group,those in the intermittent hypoxia with GBE intragastric administration group and chronic hypoxia with GBE intragastric admi