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A new experimental model to study healing process of metaphyseal fracture
  • ISSN号:0366-6999
  • 期刊名称:《中华医学杂志:英文版》
  • 时间:0
  • 分类:S858.292[农业科学—临床兽医学;农业科学—兽医学;农业科学—畜牧兽医] TH781[机械工程—仪器科学与技术;机械工程—精密仪器及机械]
  • 作者机构:[1]Peking Univ, Peoples Hosp, Dept Trauma & Orthopaed, Beijing 100044, Peoples R China, [2]First Hosp Qinhuangdao, Qinhuangdao 066000, Hebei, Peoples R China
  • 相关基金:This research was funded by grants from the Chinese National Natural Science Fund for Outstanding Youth (No.30625036),Chinese "973 Project Planning" (No.2005CB522604),National Natural Science Foundation of China (Nos.81171146,30971526,31040043),Beijing Natural Science Foundation (No.7113170),and Doctoral Fund of Ministry of Education of China (No.20090001120096).
中文摘要:

在那里的背景是为 metaphyseal 破裂愈合的很少研究;而且,没有可靠固定,学习 metaphyseal 破裂的动物模型被震荡的锯截骨术通常做,它不根据我们的当前的临床的 practice.In 这研究,我们建立了一个新模型观察 metaphyseal fractures.Methods 的愈合的过程十八只新西兰兔子在 study.The 破裂模型被使用被在兔子切开中间的胫骨的高原与压缩创造,然后重设,并且修理 screws.At 1,2,3,4,6 ,和 8 星期 po 第一,一般观察和标本的X光检查考试被做,然后他们在 methylmethacrylate 被嵌入,进有难织物 slicer.The 节的节的切割与 Giemsa 试剂被染色并且在光下面检验了有的 microscopy.Results 在所有时间的胫骨的标本的破裂排水量都不指,除了显示出 collapse.No 的,外部胼胝形成 1 星期操作能被X光检查和一般 examination.After 观察,破裂差距2 个星期手术后地,很多编织骨头被形成;从第三个星期向前,编织骨头开始变成了薄片状的骨头,和新 trabecular 结构片开始了所有到 form.In,在破裂区域形成的没有明显的 chondrocytes;因此,没有这个模型是的 endochondral ossification.Conclusions 一个理想的骨折动物模型并且对 metaphyseal 骨折 healing.The X光检查和组织学的图象的学习合适证明 metaphyseal 骨折愈合是直接骨头在次要的损伤的条件下面通过 intramembranous 骨头形成愈合的一个过程,好减小,并且坚挺的固定。

英文摘要:

Background There are few researches for the healing of metaphyseal fractures; moreover,the animal models to study the metaphyseal fractures are usually made by the oscillating saw osteotomy without reliable fixation,which is not in accordance with our current clinical practice.In this study,we established a new model to observe the healing process of metaphyseal fractures.Methods Eighteen New Zealand rabbits were used in the study.The fracture model was created by splitting the medial tibial plateau in rabbits,then reset,and fixed with compression screws.At 1,2,3,4,6,and 8 weeks postoperatively,the tibial specimens were collected; firstly,a general observation and an X-ray examination of the specimens was done,and then they were embedded in methylmethacrylate and cut into sections with hard tissue slicer.The sections were stained with Giemsa reagent and examined under light microscopy.Results There was no fracture displacement in the tibial specimens of all time points,except for one showing a collapse.No external callus formation could be observed by X-ray and general examination.After 1 week of the operation,the fracture gap was filled by mesenchymal tissue; 2 weeks postoperatively,a large number of woven bones were formed; from the third week onwards,the woven bone began to turn into lamellar bone,and new trabecular structure began to form.In all of the slices,no obvious chondrocytes formed in fracture areas; thus,there was no endochondral ossification.Conclusions This model was an ideal fracture animal model and suitable for the study of metaphyseal fracture healing.The X-ray and histological images demonstrated that metaphyseal fracture healing was a process of direct bone healing through intramembranous bone formation under the conditions of minor trauma,good reduction,and firm fixation.

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期刊信息
  • 《中华医学杂志:英文版》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中华医学会
  • 主编:
  • 地址:北京东四西大街四十二号
  • 邮编:100710
  • 邮箱:cmj@cma.org.cn
  • 电话:010-85158321
  • 国际标准刊号:ISSN:0366-6999
  • 国内统一刊号:ISSN:11-2154/R
  • 邮发代号:2-920
  • 获奖情况:
  • 1997、1998、1999年获中国科协优秀科技期刊择优资...,1992、1997年连续两年荣获全国优秀科技期刊和中国...,中国期刊方阵双高期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,荷兰文摘与引文数据库,荷兰医学文摘,美国生物医学检索系统,美国科学引文索引(扩展库),美国生物科学数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊
  • 被引量:3319