在那里的背景是为 metaphyseal 破裂愈合的很少研究;而且,没有可靠固定,学习 metaphyseal 破裂的动物模型被震荡的锯截骨术通常做,它不根据我们的当前的临床的 practice.In 这研究,我们建立了一个新模型观察 metaphyseal fractures.Methods 的愈合的过程十八只新西兰兔子在 study.The 破裂模型被使用被在兔子切开中间的胫骨的高原与压缩创造,然后重设,并且修理 screws.At 1,2,3,4,6 ,和 8 星期 po 第一,一般观察和标本的X光检查考试被做,然后他们在 methylmethacrylate 被嵌入,进有难织物 slicer.The 节的节的切割与 Giemsa 试剂被染色并且在光下面检验了有的 microscopy.Results 在所有时间的胫骨的标本的破裂排水量都不指,除了显示出 collapse.No 的,外部胼胝形成 1 星期操作能被X光检查和一般 examination.After 观察,破裂差距2 个星期手术后地,很多编织骨头被形成;从第三个星期向前,编织骨头开始变成了薄片状的骨头,和新 trabecular 结构片开始了所有到 form.In,在破裂区域形成的没有明显的 chondrocytes;因此,没有这个模型是的 endochondral ossification.Conclusions 一个理想的骨折动物模型并且对 metaphyseal 骨折 healing.The X光检查和组织学的图象的学习合适证明 metaphyseal 骨折愈合是直接骨头在次要的损伤的条件下面通过 intramembranous 骨头形成愈合的一个过程,好减小,并且坚挺的固定。
Background There are few researches for the healing of metaphyseal fractures; moreover,the animal models to study the metaphyseal fractures are usually made by the oscillating saw osteotomy without reliable fixation,which is not in accordance with our current clinical practice.In this study,we established a new model to observe the healing process of metaphyseal fractures.Methods Eighteen New Zealand rabbits were used in the study.The fracture model was created by splitting the medial tibial plateau in rabbits,then reset,and fixed with compression screws.At 1,2,3,4,6,and 8 weeks postoperatively,the tibial specimens were collected; firstly,a general observation and an X-ray examination of the specimens was done,and then they were embedded in methylmethacrylate and cut into sections with hard tissue slicer.The sections were stained with Giemsa reagent and examined under light microscopy.Results There was no fracture displacement in the tibial specimens of all time points,except for one showing a collapse.No external callus formation could be observed by X-ray and general examination.After 1 week of the operation,the fracture gap was filled by mesenchymal tissue; 2 weeks postoperatively,a large number of woven bones were formed; from the third week onwards,the woven bone began to turn into lamellar bone,and new trabecular structure began to form.In all of the slices,no obvious chondrocytes formed in fracture areas; thus,there was no endochondral ossification.Conclusions This model was an ideal fracture animal model and suitable for the study of metaphyseal fracture healing.The X-ray and histological images demonstrated that metaphyseal fracture healing was a process of direct bone healing through intramembranous bone formation under the conditions of minor trauma,good reduction,and firm fixation.