中文摘要：

目的：建立脑损伤对骨折愈合影响的动物模型,并进行稳定性评估。方法：80只成年雄性SD大鼠随机分为骨折合并脑损伤组（A组）和单纯骨折组（B组）,再按5个不同时间点（6 h、1周、2周、1个月和2个月）进行取材评估,每次取8只,损伤后观察各组的组织学、神经行为学和影像学等变化。结果：A组脑损伤后大鼠90%出现偏瘫,死亡率为10%（4/40）。脑损伤严重程度评分（neurological severity score,NSS）显示造成中度脑损伤,2周后脑损伤虽有所恢复,但NSS评分值趋于稳定。光镜下脑创伤区血脑屏障破坏,神经元广泛变性坏死。伤后1周、2周、1个月和2个月A组和B组骨痂体积测量结果分别为60.03±28.05和32.80±11.04、78.54±15.16和51.36±23.02、93.01±10.65和72.38±20.38、115.26±40.00和60.30±13.34,单位为mm3,A组和B组在2周（P=0.036）、1个月（P=0.006）和2个月（P=0.010）时骨痂体积差异均具有统计学意义;在1周时两组差异无统计学意义（P=0.065）。结论：实验制作的动物模型定量准确,确实可信,稳定性及可重复性好,是研究骨折与颅脑损伤相关性的有效平台。

英文摘要：

Objective：To establish a stable animal model for studying the effect of traumatic brain injury on bone fracture healing.Methods：Eighty adult male Sprague-Dawley rats were randomly divided into fracture combined brain injury group（A） and simple fracture group（B）.Animals of the two groups were killed 6 hours,1 week,2 weeks,1 month and 2 months after trauma,respectively.Their brain histopathology changes were observed and neurological severity scores（NSS,0 through 25 from no injury to severe injury） determined to measure the brain injury after head trauma,and fracture-healing was assessed by measuring callus volume and X ray examination at the scheduled time points after trauma.The callus volumes were compared between the groups using independent-samples t test 1 week,2 weeks,1 month and 2 months after trauma respectively.A value of P0.05 was considered statistically significant.Results：Ninety percent of the rats of group A presented with hemiplegia and the mortality rate was 10%（4/40）.The survived rats developed decorticated flexion deformity of the forelimbs,with behavioral depression,and lost some reflexes and muscle tone.The NSS were 10.83±1.94,9.33±0.82,8.17±1.17,7.83±0.75 and 8.07±0.82 with 6 hours,1 week,2 weeks,1 month and 2 months after trauma,respectively.It showed that the animals received moderate head injury,which tended to be stable from 2 weeks after trauma.Brain pathology showed that blood brain barrier was destroyed,and neurons were degenerative and necrotic at and around the trauma sites.The callus volumes（unit：mm3） of the two groups 1 week,2 weeks,1 month and 2 months after trauma were 60.03±28.05 and 32.80±11.04,78.54±15.16 and 51.36±23.02,93.01±10.65 and 72.38±20.38,115.26±40.00 and 60.30±13.34,respectively.The callus volumes of the two groups 2 weeks,1 month and 2 months after trauma were statistically and significantly different（P values were 0.036,0.006 and 0.01 respectively）,and there was no difference 1 week after trauma（P=0.065）.Conclusion?