将氯过氧化物酶固定到单壁碳纳米管修饰玻碳电极,其循环伏安曲线出现一对准可逆氧化还原峰,说明碳纳米管能够很好地促进氯过氧化物酶在电极表面的直接电子传递.该过程与溶液pH值有关,可指认氯过氧化物酶在电极表面发生的是-电子-质子传递反应.该修饰电极制作简单,性能稳定,且对氧还原具有很好的电催化效应.
The chloroperoxidase (CPO) was immobilized onto the surface of SWNTs modified electrodes. The electrochemical behaviors of modified electrode have been investigated by cyclic vohammetry. The appearance of a pair of well-defined redox peaks in the CV curve indicated that SWNTs can promote the electron transfer reaction of CPO. The results of cychc vohammetry in PBS of different pH, from 3 - 9, showed that the reaction of CPO on the modified electrode was with equal numbers of electron and protein. This readily prepared SWNTs modified electrode showed excellent stability and good catalysis to O2.