中文摘要：

使用掺合单壁碳纳米管（SWCNT）的不溶性表面活性剂双十二烷基二甲基溴化铵（DDAB）修饰玻碳电极，并将血红蛋白（Hb）固定在修饰膜中制得了稳定的固载Hb的修饰电极．循环伏安和交流阻抗测试表明，固定在电极上的Hb是一个受吸附控制的可逆电子传递过程．该氧化还原过程的CV扫描峰电位与溶液pH值成良好的线性关系，斜率39mV／pH，表明在发生Hb直接电子传递反应的同时伴随有质子参与反应．掺合SWCNT的类生物膜修饰电极较之不掺合SWCNT的修饰电极对氧气的还原具有更好的催化作用．并以SWCNT掺合量为1mg·mL^-1的DDAB修饰电极性能最佳．

英文摘要：

Hemoglobin （Hb） was immobilized onto the surface of glassy carbon electrode with the single-wall carbon nanotubes and surfactant didodecyldimethylamonium bromide（DDAB） modified previously and its electrochemical behaviors have been investigated by cyclic voltammetry. A pair of well-defined redox peaks has been obtained. Cyclic voltammetry and electrochemical impedance spectroscopy （EIS） were also used to study on the effect of different concentration of SWCNT in DDAB. The result shows that the 1 mg/mL is the best choice. The dependence of E^0＇ on solution pH with the slope of 39 mV/pH, indicated that redox of the Hb is a one electron reaction with two protons transfer. The results also showed that the immobilized Hb keeped its electrocatalytic activity toward the reduction of O2.