中文摘要：

利用气质联用仪对精制山茶油、粗制山茶油、棕榈油、废油进行组分检测分析，并进行固定化酶Novozyme435催化转酯化生产生物柴油的研究。结果显示，精制山茶油转酯化反应转化率最高。以其为原料探讨醇油物质的量比、有机溶剂、外加吸附剂等对转酯化反应转化率的影响，发现甲醇用量超过理论量后（醇油物质的量比大于3：1），转化率迅速降低。选用石油醚为反应溶剂，加入硅胶作为吸附剂，30h的转化率可从40％提高到91％。在固定床反应器中进行半连续批式运行，酶在有硅胶的系统中稳定性提高，可连续运行7批，转化率保持在82％左右；无硅胶系统中反应两批后转化率下降。

英文摘要：

The components of four tested oils - refined or rude camellia oil, palm oil and waste oil were determined by GC-MS analysis and the production of biodiesel was carried out through transesterification catalyzed by immobilized enzyme Novozyme 435.The highest conversion was ob- tained from refined tea oil as the substrate, thus the influences of the oil/alcohol molar ratio, or- ganic solvent and the addition of sorbent were further studied. The conversion was decreased be- cause of the excess methanol （oil/alcohol molar ratio〉3）. With the sherwood oil as solvent, the conversion increased from 40% to 91% in the presence of silica gel. The fixed bed bioreactor was constructed and run semicontinuously, the stability of the enzyme improved greatly in the system included silica gel. The conversion could keep at 82% after seven batches in the presence of silica gel while it decreased sharply after only two batches without silica gel.