中文摘要：

为研究塔宾曲霉（Aspergillus tubingensis）F12对类黑精及实际糖蜜酒精废水的脱色机理,考察分析了塔宾曲霉F12对类黑精模拟废水（SMW）和实际糖蜜酒精废水（MSW）的脱色进程、脱色效果、菌体吸附作用、酶活及产过氧化氢的变化等方面的异同。结果表明：塔宾曲霉F12对类黑精及实际糖蜜酒精废水均有一定的脱色作用,对SMW和MSW的最高脱色率分别为57%和36%,分别出现在脱色的第8天和第5天。两种废水培养液的pH变化趋势基本一致,还原糖的消耗与菌量的变化比较一致,推测塔宾曲霉F12对两种废水的脱色都需要还原糖,并生成类似的物质。塔宾曲霉F12在MSW中的生长情况优于在SMW中的,但对MSW的脱色率却低于对SMW的;塔宾曲霉F12对MSW的脱色过程以菌体物理吸附为主,而对SMW的脱色时物理吸附作用不大,而以生物降解为主。在F12脱色两种废水的进程中均检测到Lac（漆酶）、MnP（锰过氧化物酶）、MIP（不依赖于锰的过氧化物酶）的活力,MSW中Lac酶活高于SMW的,MSW中MIP产量也明显高于SMW的,而MnP的产酶情况则相反,SMW中MnP的产量高于MSW的,推测木质素过氧化物酶在F12脱色SMW及MSW过程中所起作用存在差异。在F12脱色两种废水的过程中均检测到H2O2的产生,H2O2对废水的脱色有贡献。综上推测,塔宾曲霉F12的脱色作用可能是吸附、产H2O2、木质素氧化酶（MnP、Lac和MIP）等的协同作用。

英文摘要：

The effects of decolarization process,the adsorption of thallus,the activity of lidin peroxidase enzyme and the production of H2O2on the decolarization of the melanoidins model wastewater（ SMW） and molasses actual wastewater（ SMW） in the presence of Aspergillus tubingensis F12,respectively,were studied in order to investigate the decolorization mechanism of melanoidins by F12.Result shows that F12 can decolor both SMW and MSW with a maximum decoloration ratio of 57%and 36%,which occured on the 8th and 5th day,respectively. The change of pH value,the depletion of reducing sugar,and dry cell weight of two effluent during decoloration were basically unanimous,indicating that the decoloration of both wastewaters by F12 requires reducing sugar and gener-ates similar products. The growth of F12 in MSW was better than that in SMW,while the decoloration of MSW was lower than SMW. In addition,the mechanism of MSW decoloration by F12 are physical adsorption,while biodegradation is the main mechanism for SMW deecoloration. Laccase,manganese-independent peroxidase（ MIP）,and manganese peroxidase（ MnP） were observed in both processes. The activity of laccase and the production of MnP in MSW waste stream were higher than that in SMW,while the yield of MIP in SMW wastestream was higher in MSW wastestream.These might attribute to the different effects of peroxidase in SMW and MSW during decoloration process. The production of H2O2,which contributes to decolorization was also observed in the both effluents. The experiment results indicate that decolorization of melanoidins and molasses spentwash by Aspergillus tubingensisi F12 may be synergistic effects of adsorption,H2O2production and peroxidase（ MnP、Lac and MIP）.