中文摘要：

研究自行筛选的一株黄曲霉（Aspergillus flavus）A5p1（保藏号CGMCC.4292）对糖蜜酒精废水（MSW）的脱色机理.在外加蔗糖情况下菌株A5p1对MSW具有较好的脱色效果,脱色率由14%增高至58%；脱色进程与细胞生长基本同步.从培养液中检测出3种木质素过氧化物酶——漆酶（Lac）及两种胞外过氧化物酶即锰过氧化物酶（MnP）和不依赖锰的过氧化物酶（MiP）的酶活,但是水平不高,认为此3种酶不是主要的脱色机制.发现由各种代谢过程产生的总H2O2生成速率与脱色率基本同步,同时在第4天达到最大值,随后下降；还原糖总消耗也在初期阶段较快.外加蔗糖后总H2O2生成速率增加10倍,达到0.0027 mmol·min-1·mL-1.认为体系中脱色机制可能与产H2O2的酶相关.紫外可见光谱分析和凝胶色谱分析表明脱色过程中有大分子物质降解.综上所述初步认为,黄曲霉A5p1脱色糖蜜酒精废水是一个受产H2O2酶影响、复杂的生物降解过程.

英文摘要：

The decolorization mechanism of molasses wastewater（ MSW） by Aspergillus flavus A5P1（ CGMCC.4292） was studied in this article. With the addition of sucrose,strain A5P1 displayed a better decolorization activity,and the decolorization rate increased from 14% to 58%. The cell dry weight was synchronized with the change of decolorization rate. Three lignin peroxidases relevant to the biodecolorization were detected in the culture fluid,including laccase and two extracellular peroxidases,a manganese-independent peroxidase（ MiP） and manganese peroxidase（ MnP）,but,with low activity. It is deducted that these three enzymes did not play a leading role in the the MSWdecolorization by the strain. The total generation rate of H2O2 from a variety of metabolic processes was found to be synchronous to the decolorization rate,both reaching maximum after 4 d and decreasing subsequently. The total reducing sugar consumption rate also appeared higher at the initial stage. The total generation rate of H2O2 increased noticeably,reaching 0.0027 mmol·min-1·mL-1in the presence of sucrose. The results of UV-visible spectrum and gel filtration chromatography indicated the degradation of large molecules during the decolorization process. Based on the above results,the decolorization process was regarded as a complex biodegradation process.