中文摘要：

介绍高效液相色谱仪的基本工作原理和仪器组成，列举了输液泵、色谱柱和几种检测器在液相色谱分析工作中常出现的技术问题和仪器故障，并对其进行分析，提出了一些解决的办法和对策。

英文摘要：

The principle and structure of high performance liquid chromatography （HPLC） was introduced in this paper. It illustrates the common problems of pump, column and detector in HPLC analysis, and gives the resolvents respectively to exchange experience with other HPLC analysts.