中文摘要：

目的探讨利用含HSPA1A（HSPT0．1）启动子荧光素酶报告基因质粒的稳定转染HepG2/HSPA1A细胞评价焦炉逸散物毒性的可行性。方法构建含HSPA1A启动子的pGL4．17／HSPA1A重组质粒并转染人HepG2细胞，建立稳定细胞株HepG2／HSPA1A。用不同浓度的炉底、炉侧和炉顶焦炉逸散物染毒HepG2／HSPA1A细胞24h，分别测定细胞的相对荧光素酶活力、存活率、丙二醛（MDA）含量、Olive尾距和微核率。结果与对照组相比，炉底焦炉逸散物染毒组细胞的相对荧光素酶活力明显升高，差异有统计学意义（P〈0．01）。且在0．15μg/L时，细胞的相对荧光素酶活力最高，为对照组的1．4倍。细胞的相对荧光素酶活力分别随着炉侧和炉顶焦炉逸散物浓度的增加而逐渐增加．差异均有统计学意义（P〈0．01）。且在最高浓度时（65．4μg／L，202μg／L）细胞的相对荧光素酶活力分别为对照组的2．1和5-3倍。仅炉底焦炉逸散物染毒后细胞的相对荧光素酶活力与MDA浓度呈正相关。差异有统计学意义（r=0．404，P〈0．05）。炉底、炉侧和炉顶焦炉逸散物染毒后，HepG2／HSPA1A细胞的相对荧光素酶活力均与Olive尾距、微核率呈正相关（r尾距分别为0．476、0．940、0．788，r微校率分别为0．580、0．649、0．432），P〈0．05。结论HepG2／HSPA1A细胞的相对荧光素酶活力可以敏感地反映焦炉逸散物的毒性效应，该细胞可用于快速评估职业环境复合污染物的综合毒性效应。

英文摘要：

Objective Using the stable HSPA1A （HSP70-1） promoter-driven luciferase reporter HepG2 cells （HepG2/HSPA1A cells） to assess the overall toxicity of coke oven emissions. Methods The stable HepG2/HSPA1A cells were treated with different concentrations of coke oven emissions （GOEs） collected from the top, side, and bottom of a coke oven battery for 24 h. After the treatments, lueiferase activity, cell viability, malondialdehyde （MDA） concentration, Olive tail moment, and micronuclei frequency were determined, respectively. Results The bottom COEs induced significant increases （P〈0.01） in relative luciferase activity up to 1.4 times the control level at 0.15 μg/L. The low dose of side GOEs （0.02 μg/L） led to a significant increase （P〈0.01） in relative luciferase activity that progressively increased to 2.1 times the control level at 65.4 μg/L. The top GOEs produced a strong dose-dependent induction of relative luciferase activity up to over 5 times the control level at the highest concentration tested （202 μg/L）. In HepG2/HSPAIA cells treated with the bottom GOEs, relative luciferase activity was positively correlated with MDA concentration （r=0.404, P〈0.05）. For the three GOEs samples, positive correlations were observed between relative luciferase activity and Olive tail moment and micronuclei frequency. Conclusion The relative lueiferase activity in HepG2/ HSPA1A cells can sensitively reflect the overall toxicity of GOEs. The stable HepG2/HSPAIA cells can be used for rapid screening of the overall toxicity of complex air pollutants in the workplace.