中文摘要：

目的：研究角膜移植后角膜新生淋巴管与新生血管间关联。方法：人角膜取自行二次角膜移植的19名患者。5’核苷酸酶-碱性磷酸酶（5＇-nase—Alkaline phosphatase．5＇-NA—ALP）双重酶组织化学染色及淋巴内皮细胞受体（lymphatic vessel endothelial receptor，LYVE—1）、内皮细胞黏附因子-1（platelet endothelial cell adhesion modecule-1，PECAM-1）双重免疫组化法标记角膜中的新生血管和淋巴管，并进行淋巴管计数（lymphatic vessels counting，LVC）和血管计数（blood vessels counting，BVC），比较BVC与LVC之间的关联。结果：角膜中存在角膜新生血管12例（63％），存在角膜新生淋巴管5例（26％）。角膜新生淋巴管仅出现在血管化角膜中。角膜移植后BVC与LVC间呈显著性正相关（r=0．725；P〈0．01）。结论：人角膜移植后角膜新生淋巴管与新生血管之间存在密切关联。

英文摘要：

AIM： To examine the relationship between corneal lymphangiogenesis and hemangiogenesis after keratoplasty. METHODS： Nineteen human corneas were obtained from 19 patients undergoing a second corneal transplantation in Zhongshan Ophthalmic Center in 2005. Blood and lymphatic vessels in human transplanted corneas were identified by lymphatic vessel endothelial receptor（LYVE- 1 ） and platelet endothelial cell adhesion modecule-1 （PECAM-1） immunohistochemistry, and double enzymehistochemistry; then the association of corneal blood vessel counting （BVC） with lymphatic vessel counting （LVC） was examined. RESULTS： Corneal hemangiogenesis was present in 12 cases （63%）, and lymphangiogenesis occurred in 5 cases （26%） of human transplanted corneas. In addition, corneal lymphangiogenesis was only present in vascularized corneas. LVC was strongly and positively correlated with BVC（ r = 0. 725 ; P〈 0.01 ）. CONCLUSION： Corneal lymphangiogenesis develops after keratoplasty, and strongly associates with hemangiogenesis.