中文摘要：

目的：microRNAs是一类非编码的小分子RNAs。通过抑制基因转录参与细胞、组织的发育、分化。本研究旨在发现调控神经发生的一些micmRNAs并探讨其分子机制，为先天性神经系统发育畸形提供理论依据及可能的治疗靶点。方法：利用MICRORNA芯片，分析E8．5天小鼠胚胎神经管来源的神经上皮细胞micmRNAs表达谱。然后利用生物信息学方法（http：／／pictar，bio．nyu．edu，http：／／www，targetscan．org）筛选相关的microRNAs并进行分析和功能预测。结果：有74个上调的基因和65个下调的microRNAs。其中一些microRNAs的靶基因是和神经系统发育相关的，例如miR～367、miR-26b、miR-10b、miR-219和-199^＊等。结论：microRNAs在早期神经系统发育中可能发挥了重要的作用。

英文摘要：

Objective： The microRNAs （microRNAs） are some small non -coding transcripts of 18 -25 nucleotides which play important roles in developmental regulation by inhibiting genetic transcription. Here, to describe microRNAs expression profiling in E8. 5d mouse neural tube. Methods： Using microarray analysis in order to explore molecule mechanism of neurogenesis and provide theory basis and potential treatment target of neural tube defect. Resutls： The data in this study showed that there were 74 up - regulated and 65 down - regulated microRNAs. Then the target genes of all these microRNAs were predicted by bio information method （ http： //pictar. bio. nym edu, http： //www. targetscan, org）. Some neural development related genes do have target site of the microRNAs （miR -367, miR-26b, miR -10b, miR- 219and so on） . Conclusion： Although the exactly function of the microRNAs need further study, this data implicates that microRNAs might play an important role in neural development in vivo.