中文摘要：

本研究旨在评价不同组合丛枝菌根真菌（arbuscular mycorrhizal fungi,AMF）抑制根结线虫和降低根结线虫病害的效应,以获得抗病作用较大的AMF组合。将由AMF光壁无梗囊霉Acaulospora laevis（Al）、摩西管柄囊霉Funneliformis mosseae（Fm）、幼套近明球囊霉Claroideoglomus etunicatum（Ce）、根内根孢囊霉Rhizophagus intraradices（Ri）、变形球囊霉Glomus versiforme（Gv）、珠状巨孢囊霉Gigaspora margarita（GIm）和全球盾巨孢囊霉Scutellospora aurigloba（SCa）组成的12个组合分别接种＂津优35号＂黄瓜,接种南方根结线虫Meloidogyne incognita并设置不接种对照。盆栽试验结果表明,接种AMF组合均能显著降低线虫卵囊数和每个卵囊的含卵数量,但Fm＋Ri＋GIm＋SCa组合则增加根内二龄幼虫数、根内雌虫数和根内线虫总数;其他大多数组合能够抑制线虫的繁殖数量,其中,以Al＋Fm＋GIm及Fm＋Ce＋Gv组合抑制线虫生长发育的作用最大;除GIm＋SCa组合外,其他AMF组合均能不同程度地降低线虫为害,防效达31%–83%,以Al＋Fm＋GIm组合处理的根结指数最低,防效最大。设施田间试验结果表明,以Al＋Fm＋GIm组合抗病作用最强。本研究表明,不同组合AMF防病效应差异显著,Al＋Fm＋Ce＋Gv组合及Al＋Fm＋GIm是本试验条件下防治根结线虫病害效应最好的AMF组合,具有较大的应用潜力。

英文摘要：

Effects of combined inoculation with various arbuscular mycorrhizal fungi（AMF） on antagonizing root knot nematode and reduction of root knot nematode disease was examined in cucumber plants grown under potted and different culture conditions. The aim of this study was to screen effective combination of AMF in enhancing plant resistance to nematode disease. Seeds of ＂Jinyou 35＂ cucumber inoculated with various combination agents of seven AMF, Acaulospora laevis（Al）, Funneliformis mosseae（Fm）, Claroideoglomus etunicatum（Ce）, Rhizophagus intraradices（Ri）, Glomus versiforme（Gv）, Gigaspora margarita（GIm） and Scutellospora aurigloba（SCa）, together with 2nd stage of juvenile nematode of Meloidogyne incognita（J2） in a pot experiment. Results showed that combined inoculation of AMF significantly reduced the egg mass numbers and egg numbers per egg mass. Inoculation of combination of Fm＋Ri＋GIm＋SCa increased the number of J2, female and total nematodes within roots; while most other combined inoculations decreased the reproduction of nematodes. Plants inoculated with combined AMF of Al＋Fm＋GIm and Fm＋Ce＋Gv exhibited the greatest inhibition to nematode. All AMF combinations except GIm＋SCa reduced the nematode damages. Al＋Fm＋GIm combination resulted in the minimum disease index, and the optimum control effect. Facility cultivation experimentals showed that Al＋Fm＋GIm combination demonstrated the best control effects. It is concluded that there are significant differences in enhancing disease control by inoculating various combination of AMF, and combinations of Al＋Fm＋Ce＋Gv and Al＋Fm＋GIm have proved to be the most effective in approving plant resistance to nematodes under the present experimental conditions, indicating their application potential.