中文摘要：

为提高杂醇油的利用率和附加值,研究非水相条件下杂醇油的脂肪酶催化酯化反应,优化其转化为天然酯类香料的工艺条件。结果表明,酶用量、分子筛用量、乙酸加入次数及间隔时间、摇床转速、反应温度、反应时间等因素条件对产物酯的生成有重要影响,采用间歇式加酸、加入适量分子筛吸水剂可明显提高混合酯得率。在正己烷10 mL、杂醇油3mL、乙酸1.5mL的反应体系中,正交试验设计优化后的工艺条件（乙酸异戊酯产率最高时）：脂肪酶Novozym435FG用量70mg、乙酸分6次加入、加入乙酸间隔时间3h、分子筛用量2g、摇床转速140r/min、反应温度45℃、反应时间36h。该条件下,乙酸异戊酯、乙酸异丁酯和乙酸丙酯的产率分别为90.1%,91.3%,78.1%。

英文摘要：

In order to improve the utilization ratio and added value of fusel oil,enzymic synthesis was studied of fusel oil into natural ester aromas using immobilized lipase Novozym 435 FG.The results showed that ester yield rates were affected on the dosage of Novozym 435 FG and molecular sieve,added times and time interval of acetic acid,shaker speed,reaction temperature and reaction time.And the ester yield rates were obviously increased using defined amount of molecular sieve and batch-added of acetic acid.In the reaction system of hexane 10 mL,fusel oil 3 mL,and acetic acid 1.5 mL,the optimized conditions by orthogonal experiment design were that Novozym 435 FG was 70 mg,acetic acid was added six times and interval time 3 h,molecular sieve was 2.0 g,shaker speed was 140 r/min,reaction temperature was 45 ℃,and reaction time was 36 h.Under the optimization conditions,the yield rates of isopentyl acetate,isobutyl acetate,propyl acetate were 90.1%,91.3% and 78.1%,respectively.