中文摘要：

目的运用微阵列芯片技术分析人脂肪源干细胞在成骨诱导分化前后miRNA表达谱的差异,并预测其靶基因。方法提取人脂肪源干细胞成骨分化前后的miRNA,分别与哺乳动物miRNA芯片V3.0杂交,应用SAM和Cluster软件进行数据分析,对有显著差异的miRNA进行实时荧光定量PCR验证,并通过miRBase数据库预测其靶基因。结果共筛选出9个差异表达miRNA,has-miR-17、has-miR-20a、has-miR-20b、has-miR-106a、has-miR-199b-5p显著上调,has-miR-125a-5p、has-miR-125b、has-miR-31、has-miR-193a-3p显著下调。结论人脂肪源干细胞成骨诱导分化前后存在明显的miRNA差异表达,预测到部分靶基因。

英文摘要：

Objective To screen the osteogenesis-specific miRNAs and forecasting target gene in human adipose-derived stem cells （ADSCs）. Methods Three pairs of microRNAs of osteo-differentiated human ADSCs were collected. miRNAs were screened by microarray technique, and analysis with SAM and Cluster software. miRNAs were confirmed by qRT-PCR. Results Total nine different miRNAs were found, among which has-miR-17, has-miR-20a, has-miR-20b, has-miR-106a, and has-miR-199b-5p were up-regulated, while has-miR-125a-5p, has-miR-125b, has-miR-31, and has-miR-193a-3p were down-regulated. Conclusions There was differential miRNAs expression during ADSCs osteo-differentiation and some of the target genes were forcasted.