中文摘要：

目的：检测IL-1β对ATDC5成软骨分化细胞miR-455-3p表达的影响，探索miR-455-3p在骨关节炎中的作用。方法诱导ATDC5细胞成软骨分化后，予10 ng/ml的IL-1β刺激，在刺激4、12、24、48 h时应用实时荧光定量PCR检测miR-455-3p、C/EBPβ和软骨特征性标记物的表达情况；并利用抑制剂IKK-NBD阻断NF-κB通路后，应用实时荧光定量PCR检测IL-1β作用下miR-455-3p的表达水平。结果在IL-1β作用下的ATDC5成软骨分化细胞中miR-455-3p、C/EBPβ和软骨退变标记物（ MMP13、ADAMTS5）均上调，而软骨基质合成标记物（ ACAN、COL2A1、SOX9）则下调，且后期更为明显；而IKK-NBD可抑制IL-1β诱导的miR-455-3p表达。结论 IL-1β可上调ATDC5成软骨分化细胞miR-455-3p的表达水平，且受NF-κB通路的调节。

英文摘要：

Objective To investigate the effects of IL-1 beta （ IL-1β） on the expression of miR-455-3p in the differentiated ATDC5 cells.Methods After chondrogenic differentiation , ATDC5 cells were treated with 10ng/ml recombinant murine IL-1βfor different time length.Additionally, a NF-kappaB inhibitor IKK-NBD was added to the cell culture before the IL-1βtreatment.The expression of miR-455-3p, C/EBP βand the genes related to cartilage were detected by quantitative real-time PCR.Results＆amp;nbsp;The IL-1β-treat differentiated ATDC5 cells increased the expression of miR-455-3p, C/EBP β, MMP13 and ADAMTS5, while decreased the expression of ACAN , COL2A1 and SOX9 especially in the late stage. IL-1β-induced up-regulation of miR-455-3p was inhibited by IKK-NBD.Conclusion The expression of miR-455-3p in the differentiated ATDC5 cells can be induced by IL-1βand NF-kappa B is involved in the up-regulation.