中文摘要：

目的：观察不同浓度Neogenin对人滋养细胞RGMa、DAPK、Neogenin表达的影响,以阐明Neogenin在滋养细胞凋亡过程中的作用。方法：先用含0,0.5,1,5,10及50ng/ml的Neogenin无血清培养基预处理TEV-1细胞24h,用噻唑蓝（MTT）比色、流式细胞术（FCM）检测细胞增殖指数及凋亡率;用实时定量PCR检测TEV-1细胞RGMa、DAPK、Neogenin mRNA的变化。结果：MTT、FCM检测表明Neogenin能诱导TEV-1凋亡,且呈一定的浓度依赖性,Neogenin对TEV-1细胞增殖无明显影响。TEV-1细胞RGMa mRNA的表达水平依次为空白对照组（0ng/ml Neogenin）的0.62±0.04,0.90±0.04,0.97±0.04,0.90±0.03及0.75±0.03;DAPK mRNA的表达水平依次为空白对照组的0.68±0.02,0.35±0.01,0.74±0.01,0.51±0.01及0.33±0.01;Neogenin mRNA的表达水平依次为空白对照组的1.45±0.03,1.80±0.05,1.22±0.08,1.47±0.00及1.14±0.00。即随着Neogenin浓度增加,TEV-1细胞内DAPK mRNA表达下调,Neogenin mRNA表达上升,RGMa mRNA的表达水平基本不变。结论：Neogenin可促进TEV-1细胞凋亡,这一过程可能是经DAPK途径调控的。

英文摘要：

Objective：To explore the underlying effect of different dose of neogenin on the expression of RGMa,DAPK and Neogenin in human trophoblast,then understand the function of neogenin in apoptosis of trophoblast.Method：TEV-1 cell were incubated in fresh media containing neogenin in various concentration（0,0.5,1,5,10 and 50ng/ml） for 24hours.TEV-1 Cell viability was measured by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide（MTT） assay and cell apoptosis were assessed by flow cytometry（FCM） assay.Real-time PCR was used to detect the mRNA expression of RGMa,DAPK and Neogenin in TEV-1 cell which cultured in vitro under different dose of neogenin.Results：The results of MTT indicated that neogenin had no effect on the proliferation of TEV-1 cell,meanwhile,the results of FCM indicated neogenin could induce the apoptosis of TEV-1 cell in a dose-dependent way.After 24 hour＇s treatment,the expression amount of RGMa mRNA varied to 0.62±0.04,0.90±0.04,0.97±0.04,0.90±0.03 and 0.75±0.03 times than that in control group;the expression amount of DAPK mRNA varied to 0.68±0.02,0.35±0.01,0.74±0.01,0.51±0.01and 0.33±0.01 times than that in the control group.The expression amount of Neogenin mRNA varied to 1.45±0.03,1.80±0.05,1.22±0.08,1.47±0.00,and 1.14±0.00 times than that in the control group.With the concentration of Neogenin increasing,the mRNA expression of DAPK was down but Neogenin was up,and the mRNA expression of RGMa was almost invariant.Conclusion：Neogenin can promote the apoptosis of TEV-1,which maybe through DAPK way.