中文摘要：

目的探讨外源性Neogenin对人滋养细胞凋亡的影响及其凋亡机制中可能存在的传导通路。方法行早孕期滋养细胞株TEV-1培养,应用吖啶橙/溴化乙啶（AO/EB）双染法检测经不同浓度的Neogenin处理细胞24 h后TEV-1细胞的凋亡情况,并同时检测Caspase-3活性的变化。结果 Neogenin可诱导TEV-1细胞凋亡,且存在剂量依赖性。在0、1、5、10及50 ng/mL浓度Neogenin作用下,其凋亡率依次为（5.34±0.32）%、（29.86±0.92）%、（38.15±0.24）%、（40.15±0.27）%及（52.37±0.20）%。但Caspase-3的活性变化不明显,依次为对照组的（1.02±0.02）、（1.06±0.00）（、0.93±0.02）（、1.04±0.01）倍。结论 Neogenin诱导滋养细胞凋亡,其凋亡信号通路与Caspase信号传导通路不存在必然联系。

英文摘要：

Objective To study the effect of Neogenin on apoptosis of trophoblast cells and the possible pathway in the mechanism of apoptosis.Methods TEV-1 cells in exponential growth stage were treated by different doses of Neogenin for 24 h.Apoptosis of TEV-1 cells was assessed by AO/EB assay.The relative Caspase-3 activity was measured by colorimetric assay.Results Neogenin could induce apoptosis of TEV-1 cells in a dose-dependent way.Under the action of different concentrations of Neogenin with 0,1,5,10 and 50 ng/mL,the apoptosis rate was（5.34±0.32）%,（29.86±0.92）%,（38.15±0.24）%,（40.15±0.27）% and（52.37±0.20）% respectively.The relative activity of Caspase-3 had no significant change and was（1.02±0.02）,（1.06±0.00）,（0.93±0.02） and（1.04±0.01） fold of that in control group.Conclusion Neogenin could induce apoptosis of TEV-1 cells without involving Caspase-transduced signal pathway.