中文摘要：

在获得暹罗鳄（Crocodylus siamensis）多肽粉的基础上,进一步研究了暹罗鳄多肽粉对羟基自由基（.OH）、超氧阴离子自由基（O2-.）和1,1-二苯基-2-苦苯肼自由基（DPPH）的清除作用以及总还原力,以揭示其抗氧化能力.同时研究了暹罗鳄多肽粉对酪氨酸酶活性抑制作用.结果显示,暹罗鳄多肽粉具有较强的抗氧化、清除自由基作用,其总还原力与暹罗鳄多肽粉含量呈正相关,对.OH、O2-.和DPPH的半清除浓度HSC50分别为1.2,2.1和6.5mg/mL.暹罗鳄多肽粉对酪氨酸酶活力有明显的抑制作用,其IC50为6.60mg/mL.结果表明,暹罗鳄多肽粉具有一定的生物学功效,是一种天然的保健品.

英文摘要：

In the previous study,we had reported that peptide powder from Crocodylus siamensis was prepared using the enzymatic catalysis of trypsin.In this paper,the effects of this peptide powder on the scavenging ·OH,O2-·,and DPPH,and its total reducing power were further investigated to reveal the antioxidation.Inhibitory effect on mushroom tyrosinase of the peptide powder was also studied.The results showed that the peptide powder had a strong antioxidant activity,with its half scavenging concentration to ·OH,O2-·,and DPPH being 1.2,2.1,and 6.5 mg/mL,respectively,and there was a positive correlation between the reducing power and its concentration.The peptide powder could strongly inhibit the biphenolase activity of mushroom tyrosinase,and the value of IC50 was determined to be 6.60 mg/mL.Our results indicate that peptide powder from C.siamensis is a natural health product,and has some potential properties in anti-fatigue and anti-aging.