中文摘要：

目的观察氟对小鼠成纤维细胞（FB）和成骨细胞（OB）c-fos mRNA和蛋白表达的影响，探讨c—fos表达改变在FB成骨功能方面的作用。方法将小鼠FB和OB分为对照组和6个染氟组，染氟（F^-）质量浓度分别为0、0．0001、0．0010、0．1000、1．0000、10．0000、20．0000mg／L，培养时间为2、4、24、48、72h。应用酶联免疫吸附法（ELISA）和RT—PCR法分别检测各时间段培养细胞上清液c—fos蛋白和染氟48h细胞中c—fosmRNA的表达。结果ELISA结果表明，各染氟组FB在各时间段c—fos蛋白水平均较相应对照组明显升高（P〈0．01）；OB c—fos蛋白水平在氟作用48h的0．0001、0．0010mg／L组（0．73±0．04、0．64±0．14）、氟作用72h的0．0001mg／L组（0．70±0．17）较对照组（0．32±0．04、0．27±0．05）明显升高（P〈0．01）。RT—PCR结果表明，染氟48h各剂量组FB c—fos mRNA表达（1．06±0．16、1．06±0．12、1．12±0．16、1．04±0．15、1．04±0．10、1.15±0．29）呈上升趋势，但与对照组（0．95±0．11）比较，差异无统计学意义（P〉0．05）；OB在染氟20．0000mg／L组c—fosmRNA表达（1．40±0．17）较对照组（1．06±0．06）明显升高（P〈0．01）。结论氟对FB和OB c—fosmRNA和蛋白表达具有明显的刺激增强作用，可能在促进FB成骨表型表达和成骨活动增强方面发挥重要作用。

英文摘要：

Objective To observe the expression of c-fos mRNA and protein in fluoride treated mouse fibroblast（FB） and osteoblast（OB） and to further explore the effects of c-fos in the osteogenic action of FB. Methods Mouse FB and OB were divided into control group and six fluoride groups （0,0.0001,0.0010,0.1000,1.0000, 10.0000,20.0000 mg/L F ）, and the levels of c-fos protein at 2,4,24,48,72 h and c-fos mRNA at 48 h were measured by using ELISA and RT-PCR methods. Results Compared with the control group, fluoride increased the content of c-fos protein obviously in all FB group（P 〈 0.01 ） ; and it is increased in 0.0001,0.0010 mg/L groups at 48 h （0.73 ± 0.04,0.64± 0.14） and 0.0001 mg/L group at 72 h（0.70 ± 0.17） in OB compared with the control group （0.32 ± 0.04,0.27±0.05, P 〈 0.01 ）. Compared with the control group（0.95 ± 0.11 ）, RT-PCR revealed an increasing tendency of the expression of c-fos mRNA at 48 h in FB （ 1.06 ± 0. 16,1.06 ±0. 12,1.12 ± 0.16, 1.04 ± 0.15,1.04 ± 0.10,1.15 ± 0.29）, but the difference was not statistically significant （P 〉 0.05）; however, a statistically significant difference（P 〈 0.01 ） of c-fos mRNA in 20.0000 mg/L group（1.40 ±0.17） in OB was found compared with the control group（ 1.06 ± 0.06 ）. Conclusion The higher expression of c-fos mRNA and protein in FB induced by fluoride may play an important role in the transformation of osteoblastic phenotype as well as increase the osteogenesis ability in FB.