采用乙醇提取法、纳氏比色法、钼锑抗显色法及F-C法分别研究了人工引种于福建省漳浦、集美、泉港、连江无瓣海桑(Sonneratia apetala)成熟叶片的生理指标:叶绿素、氮、磷及总酚含量。结果表明,4个地区叶片叶绿素a含量在(1.44±0.54)—(2.52±0.94)mg/g之间波动,叶绿素b含量范围在(0.54±0.02)—(0.94±0.11)mg/g之间,叶绿素a/b随着纬度的升高有增大趋势,表明随纬度升高无瓣海桑对蓝紫光需求比例增大;各地点无瓣海桑氮、磷含量均无显著差异,且N:P比值均小于14,表明均存在N限制;总酚含量随着纬度的升高而逐步升高,可能与无瓣海桑生长条件随纬度的上升而受到外界环境胁迫增大有关。
In this paper, we analyzed the concentration of chlorophyll, nitrogen (N), phosphorus (P), and total polyphenol of the mature leaves of Sonneratia apetala which were introduced from Lianjiang, Quangang, Jimei and Zhangpu, using ethanol extract, Nessler's Reagent Spectrophotometry, Mo anti-antimony colorimetry and Folin-Ciocalteu respectively. The concentration of chlorophyll-a ranged from (1.44±0.54) to (2.52±0.94)mg/g, and the concentration of chlorophyll-b was from (0.54±0.02) to (0.94±0.11)mg/g. The concentrations of chlorophyll-a/b increased along with the increasing of the latitude, which demonstrated that the growing demand of blue-purple light by S. apetala. There was no significant difference between nitrogen and phosphorus in 4 sample areas. The N : P ratios were less than 14, which indicates N limited exists in these four sample areas. The concentration of polyphenol increased gradually with the latitude, which could be explained by the increasing environment stress.