中文摘要：

以便为测定改进精确性在 vitro 的牛的起泡沫的病毒( BFV )，我们开发了包含编码 BFV 长终端重复倡导者驾驶的萤火虫酶的 plasmid 的一根婴儿的仓鼠肾房间( BHK ) -21-derived 指示物房间线( LTR ，从 7～1012 )。自从病毒的 trans 使活跃之物 BTas 蛋白质激活 LTR 的倡导者活动， BFV titer 能被检测酶表示决定。一克隆，指明的 BFVL，从十新霉素抵抗的克隆被选择。BFVL 响应 BFV 感染显示出一项特定、可诱导的剂量依赖者和时间依赖者酶活动。尽管在 BFVL 的酶活动的变化在 84 h 柱子感染达到顶点，区分是可能的感染并且在 48 h 的 uninfected 房间张贴感染。一种线性关系在 BFVL 在 BFV 和酶表示的激活的比率的感染(MOI ) 的复合之间被建立。而且，为检测传染 BFV 的基于 BFVL 的试金的敏感比在 48 h 柱子感染的常规基于用户终端设备的试金高 10,000 倍。这些调查结果建议基于 BFVL 的试金为 BFV 感染的察觉快速、容易、敏感、量、特定。

英文摘要：

In order to improve the accuracy for quantitating the bovine foamy virus （BFV） in vitro, we developed a baby hamster kidney cell （BHK）-21-derived indicator cell line containing a plasmid that encodes the firefly luciferase driven by the BFV long terminal repeat promoter （LTR, from -7 to 1012）. The BFV titer could be determined by detecting the luciferase expression since the viral trans-activator BTas protein activates the promoter activity of the LTR. One clone, designated BFVL, was selected from ten neomycin-resistant clones. BFVL showed a specific and inducible dose- and time-dependent luciferase activity in response to BFV infection. Although the changes in luciferase activity of BFVL peaked at 84 h post infection, it was possible to differentiate infected and uninfected cells at 48 h post infection. A linear relationship was established between the multiplicity of infection （MOI） of BFV and the activated ratio of luciferase expression in BFVL. Moreover, the sensitivity of the BFVL-based assay for detecting infectious BFV was 10,000 times higher than the conventional CPE-based assay at 48 h post infection. These findings suggest that the BFVL-based assay is rapid, easy, sensitive, quantitative and specific for detection of BFV infection.