目的考察美洲大蠊药材中油脂的提取工艺以及皂化值的测定,为今后美洲大蠊药材中油脂的测定和开发利用提供依据.方法:以石油醚为溶剂,采用正交实验设计考察物料比、提取温度、提取时间3因素对油脂的提取率和皂化值的影响.结果:采用索氏提取法,物料比为1:15,温度80 ℃,时间2 h提取率最高.结论:验证性实验结果表明,该方法操作简便、结果准确,可作为美洲大蠊药材中油脂的提取方法.
Objective Studying the extraction process of Periplaneta americana oil and determining the saponification value, toprovide scientific basis for the future oil determination,exploitation and utilization. Methods: With petroleum ether as the solvent, theeffects of extraction material ratio, temperature and time on the extraction rate and the saponification value were investigated byorthogonal experiment. Results: When using the method of cable extraction, the optimum conditions for Periplaneta americana lipidextraction were: extraction ratio of material to solvent: 1:15, temperature: 80 ℃, time: 2 h. Conclusion: Verification experimentalresults show that the method is simple and accurate, which can be used as a method for the extraction of Periplaneta americana oil.