中文摘要：

目的：建立云南琵琶甲药材的质量标准。方法：采用薄层色谱（TLC）法对云南琵琶甲中胆甾醇成分进行鉴别,按《中国药典》方法检测其重金属、水分、总灰分、酸不溶性灰分、水溶性浸出物及醇溶性浸出物含量;采用紫外分光光度法测定游离氨基酸的含量。结果：TLC斑点清晰,专属性强;重金属、水分、总灰分、酸不溶性灰分含量分别不超过百万分之十、10.0%、4.0%、0.5%,水（冷浸和热浸）浸出物、95%乙醇冷浸浸出物、95%乙醇热浸浸出物、稀乙醇冷浸浸出物、稀乙醇热浸浸出物不得少于21.0%、17.0%、20.0%、20.0%、25.0%;游离氨基酸的含量不得少于35.00mg.g-1。结论：所建标准可用于云南琵琶甲药材的质量控制。

英文摘要：

OBJECTIVE：To establish the quality standards of Balaps japanensis yunnanensis Mars.METHODS：Cholesterol was identified by TLC.The contents of heavy metals,moisture,total ash,acid-insoluble ash,water-soluble extract and alcohol-soluble extract were detected according to the standards stated in Chinese Pharmacopoeia.The content of free amino acid was determined by UV.RESULTS：The TLC sports developed were fairly clear and specific.The contents of heavy metals,moisture,total ash,acid-insoluble ash were lower than ten millionth,10.0%,4.0%,0.5%,respectively.The water extract（cold soaking and hot soaking）,95% cold ethanol extract,95% hot ethanol extract,cold homeopathic ethanol extract and hot homeopathic ethanol extract were more than 21.0%,17.0%,20.0%,20.0%,25.0%,respectively.The content of free amino acids was no less then 35.00 mg·g-1.CONCLUSION：Established method can be used for the quality control of B.japanensis yunnanensis Mars.