中文摘要：

目的：观察前成骨样细胞（MC3T3-E1细胞）分化过程中17β-雌二醇对胰岛素受体底物-1（insulinreceptorsubstrate-1，IRS-1）的影响。方法：应用10mmol／Lβ-甘油磷酸钠和50mg／L抗坏血酸诱导小鼠MC3T3-E1细胞分化成熟，分别在0，6，12，18，24，30d收集细胞。17β-雌二醇干预，用半定量RTPCR和免疫印迹法检测干预组和对照组细胞IRS-1mRNA和蛋白的表达，比较两组细胞IRS-I表达量的变化。结果：在MC3T3-E1细胞分化成熟过程中，IRS-1mRNA和蛋白的表达在MC3T3-E1细胞分化成熟过程中逐渐增高。结论：IRS-1可能在17β-雌二醇诱导的骨转换过程中起着重要作用。

英文摘要：

Objective： To observe the effect of estradiol on the expression profile of insulin receptor sub- strate-1 （IRS-1） during murine preosteoblastic MC3T3-E1 cells differentiation. Methods： MC3Td-E1 cell was cultured and underwent intervention of 17β-estradial. Cells were collected respectively in days 0, 6, 12, 18, 24 and 30. Semi-quantitative RT PCR was used to measure the expression of mRNA of IRS-1. The expression of the proteins of IRS-1 was detected by Western blotting. Results： IRS-1 mRNA and protein levels gradually increased during differentiation of MC3T3-E1 cells. Estradiol increased IRS-1 mRNA and protein expressions in the whole time course. Conclusion： IRS-1 may play an important role in the process of estradiol inducing bone turnover.