中文摘要：

目的：研究核结合因子（core binding factor alpha 1，cbfa1）的2种亚型Cbfa1／P56和Cbfa1／P57对骨髓间质细胞MBA-1凋亡的影响，并探讨其促进MBA-1凋亡的作用机制。方法：用带有cbfa1／P56或cbfa1／P57全长cDNA的质粒瞬时转染到骨髓间质细胞MBA-1，72h后用流式细胞仪观察细胞凋亡率的变化，并抽提蛋白进行Cbfa1及相关凋亡蛋白Bax，Bc1-2，cytochrome-C，caspase-3，caspase-9和TNF-α的免疫印迹检测。结果：流式细胞仪检查发现，转染Cbfa1／P56或Cbfa1／P57质粒后，MBA-1细胞的凋亡率明显增加。Western b1ot显示，Cbfa1／P56或Cbfa1／P57能增加Bax蛋白表达和Bax／Bc1-2比值，并增加cytochrome-C，caspase-3，caspase-9，TNF-α蛋白的表达。结论：Cbfa1／P56和Cbfa1／P57能诱导骨髓间质细胞MBA-1凋亡。Cbfa1促进MBA-1凋亡的机制包括线粒体途径和膜受体途径2种，凋亡相关蛋白Bax，Bc1-2，cytochrome-C，caspase-9和caspase-3组成的信号级联反应构成了Cbfa1促进MBA-1凋亡的信号转导通路，TNF-α也参加了该凋亡的信号转导。

英文摘要：

Objective To investigate the effect of two core binding factors alpha 1 （ Cbfa1 ） isfroms （Cbfa1/P56 and Cbfa1/P57） on the apoptosis of mesenchymal cell line MBA-1. Methods The two Cb-fa1 isfroms were transiently transfected into MBA-1 cells, then the changes of apoptosis rate were observed by flow cytometer. The protein expressions of Cbfa1, Bax, Bcl-2, caspase-3, and caspase-9, cytochrome-C and TNF-α were determined by Western immunoblot. Results After the transient transfection with the two isforms of Cbfa1, MBA-1, the cells apoptotic rates increased, and the ratio of Bax/Bcl-2, the expressions of cytochrome-C, caspase-3, caspase-9 , and TNF-α were significantly increased. Conclusion Cbfa1 can promote the apoptosis in mesenchymal cell line MBA-1. Bax/Bcl-2, cytochrome-C, caspase-9 , caspase-3, and TNF-α are also involved in the apoptosis pathway.