中文摘要：

目的研究胰岛素受体底物1（IRS-1）在小鼠前成骨样MC3T3-E1细胞分化过程中的表达。方法在小鼠前成骨样MC3T3-E1细胞分化成熟过程中,分别在不同时点收集细胞及上清液。骨钙素含量用放射免疫法测定;碱性磷酸酶活性用α-磷酸奈酚法测定;Ⅰ型胶原、骨涎蛋白、骨桥素和IRS-1的mRNA表达应用半定量RT-PCR检测;免疫印迹法测定IRS-1蛋白水平。结果在小鼠前成骨样MC3T3-E1细胞分化过程中,IRS-1的表达量逐渐增加,而且与Ⅰ型胶原、骨钙素、骨涎素和骨桥素呈正相关。结论 IRS-1参与MC3T3-E1细胞的增殖、成熟与矿化的全过程。

英文摘要：

Objective To investigate the expression profile of insulin receptor substrate 1（IRS-1） during the differentiation of preosteoblastic cells.Methods Pnitrophenyl phosphate assay was used to evaluate alkaline phosphatase activity,RIA was used to evaluate osteocalcin.Semi-quantitive RT-PCR was used to examine the mRNA levels of bone sialoprotein（BSP）,osteopontin（OPN）,collagen type Ⅰ（Col Ⅰ）and IRS-1 in MC3T3-E1 cells.Western blot was adopted to evaluate the level of IRS-1 protein.Results During differentiation of MC3T3-E1 cells,IRS-1 mRNA and protein levels gradually increased.Osteocalcin,Col Ⅰ,OPN and BSP were positively related to IRS-1.Conclusion IRS-1 may be involved in the proliferation,differentiation and mineralization of murine preosteoblastic MC3T3-E1 cells.