中文摘要：

目的 观察花刺参粘多糖对肿瘤坏死因子α诱导的大鼠血管平滑肌细胞血管细胞粘附分子1和细胞间粘附分子1表达的影响,以探讨花刺参粘多糖抗动脉粥样硬化的可能机制.方法组织贴块法体外原代培养大鼠血管平滑肌细胞,采用流式细胞术、逆转录聚合酶链反应和细胞粘附实验方法,观察花刺参粘多糖对肿瘤坏死因子α诱导的平滑肌细胞血管细胞粘附分子1、细胞间粘附分子1蛋白及mRNA水平表达及单核细胞与平滑肌细胞粘附的影响.结果 肿瘤坏死因子α刺激后平滑肌细胞血管细胞粘附分子1和细胞间粘附分子1表达增强,花刺参粘多糖（1.5～6.0 g/L）可从蛋白和mRNA水平浓度依赖性地抑制其表达,流式细胞分析示血管细胞粘附分子1的荧光强度由176.4±3.5减少到80.7±1.9（P＜0.01）,细胞间粘附分子1的荧光强度由92.2±2.9减少到58.3±2.1（P＜0.01）;逆转录聚合酶链反应结果发现血管细胞粘附分子1的相对光密度值由0.61±0.03减少到0.41±0.04（P＜0.01）,细胞间粘附分子1的相对光密度值由0.41±0.01减少到0.30±0.03（P＜0.05）;粘附实验发现花刺参粘多糖可减少单核细胞与平滑肌细胞粘附,光密度值由0.467±0.062减少到0.256±0.029（P＜0.05）.结论 花刺参粘多糖可抑制肿瘤坏死因子α诱导的平滑肌细胞血管细胞粘附分子1和细胞间粘附分子1的表达,减少单核细胞与平滑肌细胞的粘附和聚集,这可能对减轻血管壁的炎症反应、延缓动脉粥样硬化发生发展起一定作用.

英文摘要：

Aim To investigate the effects of Stichopus Variegates on tumor necrosis factor-alpha （TNF-α） that induced expression of vascular cell adhesion molecule-1 （VCAM-1） and intercellular adhesion molecule-1 （ICAM-1） in rat vascular smooth muscle cells （VSMC）, and to explore the mechanism of anti-athevosclerosis role of Stichopus Variegates. Methods VSMC were isolated from the media of normal rat thoracic aorta, VCAM-1 and ICAM-1 protein and mRNA expression were detected by flow cytometric technique and reverse transcription-polymerase chain reaction （ RT-PCR） respectively. Adhesion of monocytes to VSMC was measured by cell adherence assay. Results Under basal conditions of cultivation, both mRNA and protein expression of VCAM-1 and ICAM-1 in VSMC were very low. Treatment of VSMC with TNF-α （ 10μg/L） led to a markedly increase in both VCAM-1 and ICAM-1 expression （ P 〈 0.01）. Sticbopns Variegates （ 1.5 - 6.0 g/L} significantly inhibited TNF-α-in- duced protein and mRNA expression of VCAM-1 and ICAM-1. Flow cytometry showed mean fluorescence intensity of VCAM-1 decreased from 176.4±3.5 to 80.7±1.9 （P〈0.01}, ICAM-1 decreased from 92.2±2.9 to 58.3±2.1 （P〈0.01}; Relative optical density of VCAM-1 decreased from 0.61±0.03 to 0.41±0.04 analyzed by RT-PCR （ P 〈 0.01）, ICAM-1 decreased from 0.41±0.01 to 0.30±0.03）P 〈 0.05 ）. Cell adherence assay showed that Stichopns Variegates effectively reduced monocytes adhesion to VSMC, OD value decreased from 0. 467 ±0. 062 to 0. 256±0. 029 （ P 〈 0.05）. Conclusion Stichopns Variegates inhibited TNF-α-induced expression of VCAM-1, ICAM-1 and adherence of monocyte to VSMC, which may play important roles in the prevention of athevosclerosis and inflammatory response in vivo.