中文摘要：

目的：探讨右归丸对激素诱导的小鼠胸腺细胞凋亡的保护作用及机制。方法：采用Annexin V／PI双染法检测糖皮质激素及右归丸干预后小鼠胸腺细胞凋亡比例变化；同时采用RT-PCR法检测凋亡相关基因Bcl-2、BaxmRNA的表达状态。结果：激素处理小鼠的胸腺细胞凋亡比例较正常对照小鼠明显上升（P〈0．01），右归丸干预后小鼠胸腺细胞凋亡率则明显下降（P〈0．01）；同时，激素处理小鼠胸腺细胞Bcl-2的表达下调，Bcl-2／BaxmRNA比值随之下降；而右归丸干预后Bcl-2mR-NA转录水平明显高于激素处理小鼠（P〈0．01），Bax的表达虽无明显改变，Bcl-2／Bax比值较激素处理小鼠明显增高（P〈0．05）。结论：右归丸可明显抑制激素诱导的胸腺细胞凋亡，其机制与逆转激素诱导的Bcl-2／Bax表达失衡密切相关。

英文摘要：

Objective： To explore the protective mechanisms of You-gui-wan on hydrocortisone-induced apoptosis of murine thymocytes. Methods： Annexin V/PI double stain was applied to analyze the apoptotic states of thymocytes after injection with hydrocortisone coupled with or without administration You-gui-wan. The transcriptions of Bcl-2 and Bax were tested by RT-PCR. Results： The apoptotic rate of thymocytes was seriously increased in hydrocortisone-injected mice contrasted to matched control mice（ P 〈 0.01）. After administration of You-guiwan coupled with HC treatment,the apoptotic thymecytes were significantly decreased compared with hydrocortisone group （ P 〈 0.01）.Meanwhile, the expression of Bcl-2 mRNA was down-regulated by HC and resulted in reduced ratio of Bcl-2/Bax. The expression of Bcl-2 and the ratio of Bcl-2/Bax were up-regulated in You-gui-wan and HC treated mice （ P 〈0.05）. Conclusion：The results suggest that You- gui- wan can significantly protect the thymecytes from hydrocortisone-induced apoptesis, and the mechanism of protection might be partly related to regulation of the imbalance of Bcl-2/Bax.