中文摘要：

当在最高的剂量管理了或在长期的用法积累了时，类固醇荷尔蒙用作 immunosuppressor 经常导致 immunotoxicity。导致大量症状的类固醇荷尔蒙的高集中的阶段被联系到 yang 缺乏的状态，它是在繁体中文药涉及许多疾病的过程的 yin-yang 不平衡的部分。这里，我们打算由 hydrocortisone (一种类固醇荷尔蒙) 的肌内的注射在一个 yang 缺乏的动物模型在 yang 缺乏的条件下面调查 Th1/Th2-related cytokine 抄写的侧面。yang 缺乏的症状被检测活动估计，胃口，身体重量等等。T 房间增长和 cytokine 抄写被分析。自从典型 yang 缺乏的症状与减少的活动，胃口，身体重量和温度在这个模型被观察，结果证明 yang 缺乏的鼠标成功地被建立。更有趣地，在这个模型的 IFN- ， IL-2， IL-4 和 IL-10 的抄写显著地被压制，淋巴细胞的增长显著地也减少了。结果建议 yang 缺乏的症状与 cytokine 抄写和淋巴细胞增长的缺陷的导致类固醇的减小有关。因此，通过调整 cytokine 生产的新奇策略可能与 yang 缺乏症状被看作病人的有势力途径。

英文摘要：

Steroid hormone serving as an immunosuppressor often induces immunotoxicity when administered in highest dosage or accumulated in long-term usage. The stage of high concentration of steroid hormone leading to a wide range of symptoms is associated to the yang.deficient state, which is the part of yin-yang imbalance involved in processes of many diseases in traditional Chinese medicine. Here we intend to investigate the profile of Th1/Th2- related cytokine transcriptions under yang-deficient conditions in a yang-deficient animal model by intramuscular injection of hydrocortisone （a kind of steroid hormone）. The yang.deficient symptoms were estimated by detecting activity, appetite, body weight and so on. T cell proliferation and cytokine transcriptions were analyzed. The results showed that yang-deficient mice were established successfully since typical yang.deficient symptoms were observed in this model with decreased activities, appetite, body weight and temperature. More interestingly, the transcriptions of IFN-γ, IL-2, IL-4 and IL-10 in this model were markedly suppressed and the proliferation of lymphocytes significantly decreased as well. The results suggested thatyang-deficient symptoms were related to the steroid-induced reduction of cytokine transcription and impairment of lymphocyte proliferation. Therefore, novel strategies through regulating cytokine production might be considered as potent approach to patients with yang.deficiency symptoms.