中文摘要：

目的：观察结扎肠系膜淋巴管对重症失血性休克大鼠不同时期心肌自由基、炎症介质的影响。方法：雄性Wistar大鼠78只，分为假手术组、休克组、结扎组。休克组与结扎组复制重症失血性休克模型，结扎组于休克复苏后行肠系膜淋巴管结扎术。于休克后90min、输液复苏后0h、1h、3h、6h、12h、24h等时间点处死大鼠，制备心肌组织匀浆，检测MDA、SOD、TNFα、IL-6、NO、NOS以及MPO水平，RT—PCR方法测定心肌组织iNOS mRNA表达。结果：休克组大鼠输液复苏后多个时间点心肌匀浆MDA、TNFα、IL-6、MPO、NO、NOS和iNOS mRNA表达均有不同程度的升高，3h-12h持续在较高水平，均显著高于假手术组，心肌匀浆SOD活性显著低于假手术组：结扎组多个时间点心肌匀浆MDA、TNFα、IL-6、MPO、NO、NOS以及iNOS mRNA显著低于休克组相应时间点，SOD活性高于休克组相应时间点。结论：肠系膜淋巴管结扎阻断肠淋巴液回流，可减少心肌PMN扣押、降低TNFα、IL-6的释放、抑制NO生成及iNOS mRNA表达、减少自由基释放与SOD消耗。

英文摘要：

To observe the effect of mesenteric lymph duct ligation on free radical and inflammatory mediator of myocardium with severe hemorrhagic shock in rats at different period, and explore the effect of intestinal lymphatic pathway on myocardium injury patho- genesis in shock rats.Methods： 78 male Wistar rats were divided into the sham group, shock group and ligation group. The model of serious hemorrhagic shock was established in shock group, ligation group, and mesenteric lymph was blocked by ligating mesenteric lymph duct in ligation group after resuscitate. All rats were executed and taken out heart making for homogenate of 10 percent to determine the MDA, SOD, tumor necrosis factor-alpha（TNFα）, interleukin-6（IL-6）, myeloperoxidase（MPO）, NO and NOS at after shock 90 min, after transfusion and resuscitate 0 h, 1 h, 3 h, 6 h, 12 h and 24 h etc. different times, and the expression of inducible nitric oxide synthase（iNOS） mRNA in myocardium was detected by RT-PCR.Results： The contents of MDA, TNFα, IL-6, MPO, NO, NOS and iNOS expression in myocardium of shock group were rising after transfusion and resuscitate, and that was higher level at 3 h to 12 h, and that was significantly higher than sham group, the activity of SOD was significantly lower than sham group. The contents of MDA, TNFα, IL-6, MPO, NO, NOS and iNOS expression in myocardium of ligation group were significantly lower than that of shock group at sameness points, and the SOD activity was higher.Conclusion： The mesenteric lymph duct ligation and blocking mesenteric lymph could reduce the PMN detaining, decrease the discharging of TNFα and IL-6, reduce the NO and expression of iNOS mRNA, and reduce the releasing of free radical and consuming of SOD.