中文摘要：

目的：探讨肠源性内毒素移位在肠淋巴再灌注（MLR）加剧肠系膜上动脉闭塞性（SMAO）休克多器官损伤中的作用与机制。方法：24只Wistar大鼠随机分为4组（n=6）：假手术组（Sham,仅麻醉与手术）、MLR组（夹闭肠系膜淋巴管1 h,再灌注2 h）、SMAO组（夹闭肠系膜上动脉1 h,再灌注2 h）和SMAO＋MLR组（同时夹闭肠系膜淋巴管和肠系膜上动脉1 h,再灌注2 h）。再灌注2 h后,腹主动脉取血,制备血浆;留取固定位置的肝、肾、心肌、肺组织,制备组织匀浆。应用鲎试剂动态浊度法检测血浆以及各组织匀浆内毒素（ET）含量;应用酶联免疫方法检测各器官组织匀浆脂多糖结合蛋白（LBP）、脂多糖受体（CD14）和肿瘤坏死因子（TNF-α）水平。结果：Sham组和MLR组各指标均无统计学差异;SMAO组及SMAO＋MLR组的血浆、肝、肾、心肌、肺组织匀浆的ET含量均显著高于Sham组和MLR组,且SMAO＋MLR组血浆及各组织匀浆的ET水平均显著高于SMAO组;SMAO组及SMAO＋MLR组肝、肾、心肌、肺组织匀浆CD14、LBP和TNF-α水平显著高于Sham组及MLR组,且SMAO＋MLR组各指标均高于SMAO组。结论：MLR加剧SMAO休克多器官损伤的作用机制与ET经过肠淋巴途径移位、激活内毒素增敏系统LBP/CD14、促进炎症反应有关。

英文摘要：

Objective： To investigate the role of mesenteric lymph reperfusion（MLR） aggravates multiple organs injury in superior mesenteric artery occlusion（SMAO） shock and its mechanism.Methods： Twenty four Wistar rats were randomly divided into 4 groups（n=6）： Sham group（only anesthetized and operated）,MLR group rats performed 1 h occlusion of mesenteric lymph duct（MLD）,then followed by 2 h of reperfusion,SMAO group（rats performed 1 h occlusion of superior mesenteric artery（SMA） and then followed by 2 h of reperfusion）,SMAO＋MLR group（rats performed 1 h occlusion of SMA and MLD and then followed by 2 h of reperfusion）.The blood sample was taken out from abdominal aortic for plasma and the liver,kidney,myocardium,lung tissues in fixed position were prepared for making homogenate after reperfusion of 2 h respectively.And the levels of endotonxin（ET） in plasma and homogenates were determined with kinetic turbidimetric technique of tachypleus amebocyte lysate,the contents of lipopolysaccharide-binding protein（LBP）,lipopolysaccharide receptor（CD14） and tumor necrosis factor-α（TNF-α） in homogenates were determined with enzyme-linked immunosorbent assay method.Results： The indices have no statistics difference between sham group and MLR group.The ET levels of the plasma and hepatic,renal,myocardial,pulmonary homogenates in SMAO and SMAO＋MLR groups were significant higher than that of sham and MLR groups,and these indices in SMAO＋MLR were increased significantly than those in SMAO group.The CD14,LBP and TNF-α contents of the hepatic,renal,myocardial and pulmonary homogenates in SMAO and SMAO＋MLR groups were significant higher than those in sham and MLR groups,and these indices in SMAO＋MLR were higher than SMAO group significantly.Conclusion： The mechanism of MLR aggravates multiple organs injury in SMAO shock may be associated with enterogenous ET through intestinal lymphatic pathway to translocate,activate the LBP/CD14 as endotoxin sensitizing system and promot