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利用拓扑异构酶Ⅱ提高转化效率及其在从细菌群落中克隆六六六γ异构体脱氯化氢酶基因中的应用
  • ISSN号：0253-2654
  • 期刊名称：《微生物学通报》
  • 时间：0
  • 分类：Q785[生物学—分子生物学]
  • 作者机构：[1]南京大学环境学院污染与资源化国家重点实验室,江苏南京210014
  • 相关基金：The National Natural Science Foundation（No.20637030）; The National Science and Technology Surporting Item（No.2006BAJ08B01-02）; The Science and Technology Department of Jiangsu Province of The People’s Republic of China（No.BS2007160）
作者： 李丽观[1], 杜宏伟[1], 王晓琳[1], 蒋丽娟[1], 杨柳燕[1], 武俊[1]
关键词： 拓扑异构酶Ⅱ, T-A重组分子, 脱氯化氢酶基因, Topoisomerase II, T-A recombinant molecules, linA-like
中文摘要：

一个简单的技术上的调整被应用于利用T载体构建的基因组或宏基因组文库策略中。该技术利用拓扑异构酶Ⅱ将超螺旋引入松散的T-A环状重组分子,从而提高转化效率。利用该技术构建一个宏基因组文库,并从中克隆到一个六六六γ异构体脱氯化氢酶基因（linA-like）。

英文摘要：

A simple modification was developed for construction of genomic/metagenomic libraries by T-vectors.Topoisomerase II was employed to introduce superhelical turns into relaxed circular T-A recom-binant molecules,which greatly increased the transformation efficiency.By using this technique,a metage-nomic library was constructed and a γ-hexachlorocyclohexane dehydrochlorinase gene linA-like was screened from it.

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期刊信息
  • 《微生物学通报》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院微生物研究所 中国微生物学会
  • 主编：赫荣乔
  • 地址：北京市朝阳区北辰西路一号院3号中国科学院微生物研究所内B401室
  • 邮编：100101
  • 邮箱：xuj@sun.im.ac.cn
  • 电话：010-64807511
  • 国际标准刊号：ISSN：0253-2654
  • 国内统一刊号：ISSN：11-1996/Q
  • 邮发代号:2-817
  • 获奖情况:
  • 1992年中国科学技术协会优秀学术期刊三等奖,1992年国家科委中共中央宣传部新闻出版署“全国优...,2000年中国科学院优秀期刊三等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,波兰哥白尼索引,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2011版）,中国北大核心期刊（2014版）,中国北大核心期刊（2000版）
  • 被引量:29487