中文摘要：

采用变性梯度凝胶电泳（DGGE）和寡聚核苷酸探针-荧光原位杂交（FISH）方法研究了十溴联苯醚（BDE209）对土壤中微生物群落的影响作用。结果表明，1mg·kg^-1 BDE209对土壤中微生物群落多样性以及总细菌数有明显的促进作用，土壤中的氨氧化细菌以及亚硝酸氧化菌的数量有显著增长。BDE209浓度达到100mg·kg^-1时，土壤微生物的总数和群落多样性则显著降低，同时氨氧化细菌以及亚硝酸氧化菌的生长也受到明显的抑制。暗室培养45d内，BDE209虽未被降解，但高浓度的十溴联苯醚会对土壤中的微生物群落结构及土壤潜在的硝化作用产生较大影响。

英文摘要：

PBDEs are ubiquitously used fire retardant in the world and sink in the soils and sediments because of their hydrophobia characteristics. In this study, soils were treated with 1, 10 and 100 mg.kg^-1 BDE 209 for up to 45 days. The effects of BDE209 on soil bacterial community composition were studied using the denaturing gradient gel electrophoresis（ DGGE ）. The effects of BDE209 on the ammonia-oxidizing bacteria （AOB） and the nitrite-oxidizing bacteria （NOB） groups were evaluated using fluorescence in situ hybridization （FISH） analysis. The results indicated that microbial communities were significantly affected by BDE209. After 45 days treatment, soil microbial community diversity and the amount of AOB and NOB could be promoted obviously by BDE209 at the concentration of 1 mg.kg^-1, but inhibited at 100 mg.kg^-1. BDE209 was not degraded under darkness throughout the experiment. These observations demonstrated that BDE209 in soil, although at low bioavailability state, had an adverse impact on the soil microbial community structure and nitrification potential.