中文摘要：

目的研究FKBP25缺失突变体蛋白在细胞内的表达和定位。方法以人FKBP25全长cDNA序列的质粒为模板，PCR方法分别扩增出FKBP25缺失突变体序列，然后插入真核表达载体peDNA3．I中，通过Westernblot方法和免疫荧光方法检测其在细胞株中的表达和定位。结果成功构建了FKBP25缺失突变体的真核表达载体，经Westernblot检测FKBP25缺失突变体在HEK293T细胞中能够有效表达，免疫荧光显微镜结果显示FKBP25缺失突变体在COS7细胞中分布于细胞核和细胞质。结论FKBP25缺失突变体住HEK293rr、COS7细胞中能够表达，为了解FKBP25缺失突变体的功能提供了一定基础。

英文摘要：

Objective To investigate the expression and localization of the product of FKBP25 deleted mutants in cells. Methods The full length eDNA fragment of FKBP25 was used for PCR amplification, to construct FKBP25 deletion mutant gene sequence respectively, and inserted them into pcDNA3.1 vector. All expressions were detected by Western blot and immunofluorescenee. Results The expression of FKBP25 deletion mutants was constructed successfully. Western blot assay showed that FKBP25 deletion mutants could express efficiently in HEK293T cell line, and the results of immunofluorescence microscopy showed that the FKBP25 deletion mutants protein was found to be localized in the nucleus and cytoplasm in COS7 cells lines. Conclusion The FKBP＇25 deletion mutants can express in HEK 293T and COS7 cell lines. The results provide the basis for further understanding of the function of FKBP25 deletion mutants.