中文摘要：

以含CDNA序列的质粒为模板，下游引物带有FLAG标签，PCR扩增，插入pcDNA3．1，成功构建C端带FLAG标签的人野生型肽基脯氨酰顺反异构酶FKBP3的哺乳动物细胞表达载体pcDNA3．1-FKBP3-FLAG。转染HEK293T细胞，Western blot检测；转染COS-7细胞，荧光显微镜观察。FKBP3在HEK293T、COS-7细胞中均有表达，且定位在COS-7细胞的细胞质和细胞核，胞质分布较多，为进一步研究FKBP3的相互作用蛋白和功能奠定了一定的基础。

英文摘要：

The full length cDNA fragment of FKBP 3 from plasmid was used for PCR amplification with FLAG tag inthe downstream primer. The PCR product was inserted into pcDNA3.1 vector, to construct the mammalian cell expression vector of wild type FKBP3 with FLAG tag in C-terminal pcDNA3.1-FKBP3-FLAG. Its expression was detected in transfected HEK 293T cells with Western blot. The localization of FKBP3 protein in transfected COS-7 cells was observed by immunofluorescence. The transfected ceils can express FKBP3-FLAG efficiently in the two cell lines, and the FKBP3 protein was found to localize in both cytoplasm and nucleus in COS-7 cell line, with majority in the cytoplasm. The results provide the basis for further studying of the function and interaction partners of FKBP3 protein.