中文摘要：

采用活性追踪分离的方法，从鱼藤中分离鉴定出了2个抗肿瘤活性化合物，即羟基鱼藤素（1）和鱼藤酮（2）．用丽丝胺罗丹明B（SRB）法评价其抗肿瘤活性，用紫外光谱和荧光光谱等方法探讨了化合物与DNA的相互作用．结果发现，羟基鱼藤素（1）与鱼藤酮（2）为鱼藤的抗肿瘤活性成分，对人大肠癌细胞HCT8、人肝癌细胞BEL7402、人胃癌细胞BGC823、人肺癌细胞A549和人卵巢癌细胞A2780的半数抑制浓度（IC50）在0．1～90．0μmol／L之间．这两种化合物的紫外吸收强度随着HS—DNA的加入均呈现减色效应，并使EB—HS—DNA复合体系荧光强度减弱，表明化合物的抗肿瘤活性与DNA以插入方式作用有关．

英文摘要：

Two antitumor compounds, tephrosin（1） and rotenone（2）, were acquied by bioassay-guided isolation from Derris trifoliate Lour. Compounds 1 and 2, assayed by SRB （Sulforhodamine B） methods, showed antitumor activities against cell lines of HCT8, BELT402, BGC823, A549 and A2780 in vitro with IC50 values from 0. 1 to 90. 0 μmol/L. The interaction of the compounds with HS-DNA was investigated using UV spectra and fluorescent spectra. The absorbance values at the UV max peaks of compounds 1 and 2 were reduced by adding HS-DNA while the intensity of fluorescent spectra of EB-HS-DNA were gradually weaken following the increased amounts of compounds 1 and 2. It indicated that the mode of antitumor activities of compounds 1 and 2 was relative to binding with DNA through intercalation mode.