褐藻胶主要来源于褐藻细胞壁,由L-古罗糖醛酸和D-甘露糖醛酸通过1→4糖苷键连接形成直链大分子,含有聚甘露糖醛酸片段、聚古罗糖醛酸片段和聚甘露糖醛酸-古罗糖醛酸杂合段.大分子褐藻胶具有抗肿瘤、增强免疫等活性,分子中的甘露糖醛酸是主要酌激发因子.褐藻胶经降解获得的寡糖能显著提高生物活性并赋予新的生物活性.褐藻胶用酶法降解在寡糖非还原端形成不饱和双键。
A series of oligomannuronates with series degree of polymerization ( DP = 2--8) were prepared by acid hydrolysis from alginate-derived homopolymannuronate blocks, and the structures of the oligosaccharides were analyzed. The macromolecule of alginate was treated by partial acid hydrolysis and fractionated at pH = 2.85 resulting in homopolymannuronate blocks. The homopolymer was degraded by acid hydrolysis, and the oligosaccharides were separated and purified by gel chromatography. The oligosaccharides were characterized by fluorophore-assisted carbohydrate electrophoresis(FACE) , and the structures of derived oligomannuronates were determined by ESI-MS, NMR as well as FTIR analysis.