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系列甘露糖醛酸寡糖的制备与鉴定
  • 期刊名称:刘斌, 王长云*, 张洪荣, 王斌贵, 魏玉西, 康凯, 李亮, 于广利, 管华诗, 耿美玉. 系
  • 时间:0
  • 分类:O62[理学—有机化学;理学—化学]
  • 作者机构:[1]中国海洋大学海洋药物研究所、海洋药物教育部重点实验室,青岛266003, [2]中国科学院海洋研究所实验海洋生物学重点实验室,青岛266071, [3]青岛大学生物系,青岛266071
  • 相关基金:国家“九七三”计划(批准号:2003CB716400)、国家“八六三”计划(批准号:2004AA621020)、山东省优秀中青年科学家科研奖励基金(批准号:03BS109)和中国科学院海洋研究所实验海洋生物学重点实验室开放基金(2004)资助.
  • 相关项目:中药复方海藻制剂抗肿瘤活性成分及其作用机制研究
中文摘要:

褐藻胶主要来源于褐藻细胞壁,由L-古罗糖醛酸和D-甘露糖醛酸通过1→4糖苷键连接形成直链大分子,含有聚甘露糖醛酸片段、聚古罗糖醛酸片段和聚甘露糖醛酸-古罗糖醛酸杂合段.大分子褐藻胶具有抗肿瘤、增强免疫等活性,分子中的甘露糖醛酸是主要酌激发因子.褐藻胶经降解获得的寡糖能显著提高生物活性并赋予新的生物活性.褐藻胶用酶法降解在寡糖非还原端形成不饱和双键。

英文摘要:

A series of oligomannuronates with series degree of polymerization ( DP = 2--8) were prepared by acid hydrolysis from alginate-derived homopolymannuronate blocks, and the structures of the oligosaccharides were analyzed. The macromolecule of alginate was treated by partial acid hydrolysis and fractionated at pH = 2.85 resulting in homopolymannuronate blocks. The homopolymer was degraded by acid hydrolysis, and the oligosaccharides were separated and purified by gel chromatography. The oligosaccharides were characterized by fluorophore-assisted carbohydrate electrophoresis(FACE) , and the structures of derived oligomannuronates were determined by ESI-MS, NMR as well as FTIR analysis.

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