中文摘要：

为测定麦角固醇和维生素D2及其他光化学异构体，研究确定了HPLC—ELSD测定的色谱分离条件：KromasilC18柱（4．6×250mm，5μm），流动相为体积比95：5甲醇-水溶液，流速1．3ml／min，蒸发光散射检测器漂移管温度为75℃，气体流量1．6L／min。在此条件下，各组分得到有效地分离，麦角固醇和维生素D2色谱峰面积的自然对数与浓度的自然对数呈良好的线性关系（r〉0．997），精密度RSD为1．89％～2．75％，最低检测限为0．05--0．1μg，回收率测定值为101．4％～102．8％。

英文摘要：

An I-IPLC-ELSD method for the determination of ergosterol and the photochemical iso- mers of vitamin I32 were studied. Chromatographic analysis was carried out on a Kromasil C18 column（4.6 mm×250mm,5μm） with methanol-water （95：5,V/V）as the mobile phase. The flow rate was 1.3 ml/min. The drift tube temperature was at 75℃ and gas flow rate was 1. 6SLPM. The relative standard deviation （RSD） was below 2.75 % （ n = 6）, and detection limits attainable were in the range 0.05μg for ergosterol to 0.1μg for vitamin D2, and the recoveries were from 101.4 % to 102.8 %.