中文摘要：

本研究主要探讨转染方法对不同细胞系的转染效果。实验采用电穿孔和脂质体转染方法，将线性化的绵羊肌肉生长抑制素基因（myostatin）药物正负筛选（PNS）打靶载体pLoxp-1．4K-4．3KDNA导人体外培养的绵羊胎儿和成年绵羊耳部成纤维细胞中，而后采用G418和GANC进行药物抗性细胞克隆筛选和培养。结果发现，脂质体法对myostatin基因敲除载体的转染效果明显优于电击法，但电穿孔法对细胞类型的依赖性较小；采用与载体基因型相同的细胞系，两种转染方法的转染效率均略高于对照成年绵羊成纤维细胞系。

英文摘要：

The transfecting efficiency of electroporation and lipofectamin on different fibroblasts cultured in vitro was compared in this study. Sheep fetal and adult fibroblasts were transfected with sheep myostatin replacement vector pLoxp-1.4K-4.3K by either electroporation or lipofectamin, then cultured in G418＋/GANC＋ drug selection medium. Calculated from the number of G418＋/GANC＋ resistant cell clone, the efficiency of lipofectamin transfection method was higher than that of the electroporation, however, the transfecting efficiency of electroporation was independent of cell type. The efficiency of DF1 cell lines （the genome identical with the constructed vector） was higher than that of the DF2 （the common fibroblasts） in the two methods.