中文摘要：

目的：检测不同浓度TNF-对体外培养人牙囊细胞增殖活性和碱性磷酸酶活性的影响。方法：第5代人牙囊细胞接种于96孔培养板上，分别与不同浓度的TNF-共同孵育，检测TNF-对人牙囊细胞的增殖活性和碱性磷酸酶活性的影响。结果：TNF-在浓度为10-50ng／ml，时间为3天时促进人牙囊细胞的增殖，浓度为l0-200ng／ml时抑制碱性磷酸酶活性。结论：TNF-在特定的浓度和时间促进人牙囊细胞的增殖，抑制牙囊细胞的成骨特性。

英文摘要：

Objective To study the effects of different concentration TNF-a on the activity of proliferation and the activity of alkaline phosphatase of human dental follicle cells. Methods The 5th passage human dental follicle cells were seeded on 96-well plates and co-cultured with different concentration of TNF-α, the activity of proliferation and the activity of alkaline phosphatase was assayed. Results The 10N50ng/ml TNF-a enhanced the activity of proliferation profoundly and the effects were significant at 3 days after coculture. 10N200ng/ml TNF-α decreased the activity of alkaline phosphatase. Conclusions TNF-α enhance the activity of proliferation and decreased the activity of alkaline phosphatase of human dental follicle cells at special concentration and time.