中文摘要：

目的：研究白细胞介素-10（Interleukin-10，IL-10）对体外培养人牙囊细胞增殖和牙囊细胞碱性磷酸酶活性的影响。方法：体外培养人牙囊细胞，取生长状态良好的第5代细胞，用四甲基偶氮唑蓝法（MTF法）检测IL-10对细胞增殖的作用；用碱性磷酸酶试剂盒检测IL-10及其抑制剂对细胞碱性磷酸酶活性的作用。结果：0、1、10、25、50、100rig／mL IL-10对人牙囊细胞的增殖影响无显著性差异（P〉0．05）；10mg／mL IL-10作用0、1、3、5、7d对人牙囊细胞的增殖影响无显著性差异（P〉0．05）。10mg／mLIL-10作用3～7d降低人牙囊细胞的碱性磷酸酶活性（P〈0．05），10ng／mL IL-10抑制剂作用5～7d增加人牙囊细胞的碱性磷酸酶活性（P〈0．05）。结论：IL—10对人牙囊细胞的增殖无影响。IL-10降低人牙囊细胞的碱性磷酸酶活性，说明IL—10抑制人牙囊细胞向成骨方向分化。

英文摘要：

AIM： To study the effect ofinterleukin- 10 （IL- 10） on the proliferation of human dental folli- cle cells （HDFCs） and the effects of IL - 10 and its inhibitor on the activity of alkaline phosphatase （ALP） of HDFCs. METHODS： HDFCs of the 5th passage were cocuhured with IL - 10 for the assay of proliferation. In addition, cells were co -cultured with IL - 10 or its inhibitor for the assay of activity of ALP. RESULTS： 0, 1, 10, 25, 50 or 100ng/mL IL - 10 had no significant difference on the proliferation of HDFCs（ P 〉 0.05 ）. 10ng/mL IL - 10 acting for 0d, 1 d, 3 ds, 5 ds or 7ds had no significant difference on the proliferation of HDFCs （ P 〉 0.05 ）. 10ng/mL IL - 10 act- ing for 3 -7 days decreased the activity of ALP（P 〈0.05）. 10ng/mL IL- 10 inhibitor acting for 5 -7days increased the activity of ALP（ P 〈 0.05）. CONCLUSION ： IL - 10 had no effect on the proliferation of HDFCs. However, IL - 10 suppressed the activity of ALP of HDFCs, inhibiting their differentiation to osteoblasts.