中文摘要：

目的：研究巨噬细胞集落刺激因子对体外培养的人牙囊细胞破骨细胞分化因子（RANKL）mRNA表达的影响。方法：原代培养人牙囊细胞，传至第3代，制备细胞爬片，进行RANKL免疫组化染色；选取生长状态良好的第3-6代人牙囊细胞，胰酶消化后制成单细胞悬液，培养基中分别加入25ng／mL的巨噬细胞集落刺激因子，共同孵育0．5、1、3、6、12h，提取总RNA进行RT-PCR，灰度值半定量分析RANKL mRNA的变化。结果：正常人牙囊细胞RANKL蛋白表达阳性；25ng／mL的巨噬细胞集落刺激因子可升高人牙囊细胞RANKL的表达；共同孵育3h，RANKL表达最高。结论：巨噬细胞集落刺激因子对体外培养的人牙囊细胞表达RANKL，具有正向调节作用。

英文摘要：

AIM： To study the effects of colony - stimulating factor - 1 （ CSF - 1 ） on the expression of osteoclast differentiation factor （RANKL） mRNA in human dental follicle cells. METHODS：The culture of human dental follicle cells （HFDC） was established and the cells growing on coverslips were stained by RANKL antibody immunohistochemically, The fourth - passage human dental follicle cells were incubated with 25 ng/mL of CSF - 1 for 0, 5,1,3,6 and 12 h ,then the cells were collected for RT -PCR assays. RESULTS ：This study shows that the RANKL was possitively expressal on human dental follicle cells and 25 ng/mL of CSF - 1 up - regulates the expression of RANKL mRNA, CONCLUSION：RANKL is expressed in human dental follicle cells and the gene expression is enhanced by CSF- 1.