中文摘要：

目的探讨脑梗死患者经丹参注射液诱寻重组人粒细胞集落刺激因子（rhG—CSF）动员自体外周血干细胞后，外周血脑源性神经生长因子（BDNF）表达情况。方法将60例14d后40岁～60岁发病14d后脑梗死患者分为丹参注射液＋rhG—CSF组、rhG—CSF组、丹参组、常规组，每组15例。常规组予常规治疗；丹参组：丹参注射液20mL／d静脉输注，连用7d，余治疗同常规组。rhG—CSF组：在抽血检查次日08：00皮下注射古粒芬300μg，连用3d，余治疗同常规组。丹参＋rhG—CSF组：第1天开始连续使用丹参注射液20mL／d静脉输注7d，同时在丹参注射液滴注第2天08：00皮下注射rhG—CSF300μg，连用3d，余治疗同常规组。4组均以7d为1个疗程。Elisa法检测外周血各组BDNF含量。结果丹参＋rhG—CSF组及rhG—CSF组治疗后外周血BDNF表达分别为8．944ng／mL±1．932ng／ITIL，8．684ng／mL±1．412ng／mL，呈上升趋势，与丹参注射液组及空白组（7．874ng／mL±1．934ng／mL，7．309ng／mL±1．779ng／mL）相比，差异有统计学意义（P〈0．05）。结论丹参注射液配伍rh—CSF以及rh—CSF本身能够提高脑梗死患者外周血BDNF的表达，可能对脑梗死患者神经功能修复有一定的促进作用。

英文摘要：

Objective To explore the effect of Danshen injection on brain - derived neurotrophic factor （BDNF） expression paracrine - secreted by autologous peripheral blood stem cells mobilization using recombinant human granulocyte colony - stimulating factor （rhG- CSF） in patients with cerebral infarction. Methods Sixty patients with onset of 14 days after cerebral infarction were divided randomly into four groups：Control group, rhG - CSF group given rhG - CSF 300 μg/d subcutaneously for consecutive 3 d at the next day after blood tests,Danshen injection group given Danshen injection 20 mL/d intravenously for 7 d, Danshen injection plus rhG- CSF group. There was 7 d for a course. The expression of BDNF in peripheral blood was detected by ELISA. Results After treatment, the expression of BDNF in peripheral blood were increased in Danshen injection plus rhG- CSF group（8. 944 ng/mL± 1. 932 ng/mL） and in rhG - CSF group（8. 684 ng/mL± 1. 412 ng/mL）, which were higher than that in Danshen injection group（7. 874 ng/mL±1. 934 ng/mL） and control group（7. 309 ng/mL±1. 779 ng/mL,P〈0.05） ,respectively. Conclusion Danshen injection plus rh-CSF or rh - CSF could upregulate the expression of BDNF in peripheral blood,which could promote recovery of nerve function in patients with cerebral infarction.